[1]梁秀文*,季新梅,马娟.多囊卵巢综合征患者外周血单个核细胞中RPS 26 及PPARγ基因的表达[J].中国计划生育和妇产科,2017,(1):55-58.
　LIANG Xiu-wen*,JI Xin-mei,MA-juan.Expression of RPS 26 and PPARγ gene in peripheral blood mononuclear cells of patients with polycystic ovary syndrome[J].Chinese Journal of Family Planning & Gynecotokology,2017,(1):55-58.
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多囊卵巢综合征患者外周血单个核细胞中RPS 26 及PPARγ基因的表达
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2017年1期
页码:
55-58
栏目:
论著与临床
出版日期:
2017-01-25

文章信息/Info

Title:
Expression of RPS 26 and PPARγ gene in peripheral blood mononuclear cells of patients with polycystic ovary syndrome
作者:
梁秀文*; 季新梅; 马娟
深圳市龙华新区人民医院妇科
Author(s):
LIANG Xiu-wen*; JI Xin-mei; MA-juan
Department of Gynecology, People’s Hospital of Longhua New District, Shenzhen Guangdong 528100, P.R.China.
关键词:
PCOS; 外周血单个核细胞; RPS 26; PPARγ; 表达
Keywords:
PCOS; PBMNC; RPS 26; PPARγ; expression
摘要:
目的探讨外周血单个核细胞（peripheral blood mononuclear cell，PBMNC）中核糖体蛋白(ribosonoal protein S 26，RPS 26)和卵巢颗粒细胞中过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor gamma，PPARγ)基因表达与多囊卵巢综合征（polycystic ovary syndrome，PCOS）患者病情的相关性。方法将深圳市龙华新区人民医院妇科2015年7月至2016年3月诊治的38例PCOS患者作为PCOS组，同期体检健康女性30例作为对照组，抽取外周血，采用Ct值比较法，通过SYBR Green II 实时定量PCR检测两组PBMNC中RPS 26及PPARγ基因在mRNA的表达水平，进一步分析PCOS患者两基因表达水平及与体质量指数（body mass index，BMI）、性激素水平相关性。结果PCOS组RPS 26表达水平高于对照组，但差异无统计学意义（P＞005），PCOS组PPARγ基因表达明显高于对照组，差异有统计学意义（P＜005）。RPS 26及PPARγ基因表达水平与睾酮呈正相关（P＜005），与促卵泡成熟素呈负相关（P＜005）。结论RPS 26及PPARγ基因在PCOS患者PBMNC中的表达均高于正常女性，可不同程度地反映疾病的严重程度。
Abstract:
ObjectiveTo investigate correlation of the expression of RPS 26 and PPARγ in peripheral blood mononuclear cells and condition of patients with PCOS. Methods38 cases of PCOS patients and 30 cases of healthy women as control were collected in People’s Hospital of Longhua New District from July 2015 to March 2016. Peripheral blood was drawn and detected with CT value comparison method. RPS 26 and PPARγ gene expression in peripheral blood single mononuclear cells (PBMNC) level was analyzed by SYBR Green II real-time quantitative PCR with further analysis of correlation in gene expression levels of PCOS patients,their body mass index （BMI）and gonadal hormone level. ResultsThe expression level of RPS 26 in PCOS group was higher than that in control group, but with no statistical difference (P>005). The expression of PPARγ in PCOS group was significantly higher than that in control group, with statistical difference (P<005). RPS 26 and PPARγ gamma gene expression were significantly positively correlated (P<005). RPS 26 and PPARγ gene expression levels were positively correlated with testosterone(P<005), and negatively correlated with follicle-stimulating hormone(FSH) (P<005). ConclusionThe expression of RPS 26 and PPAR gene in PBMNC of patients with PCOS are higher than those in normal women, and correlated with the severity of disease.

参考文献/References:

［1］Zhao H, Lv Y, Li L, et al. Genetic Studies on Polycystic Ovary Syndrome ［J］. Best Practice & Research Clinical Obstetrics & Gynaecology, 2016，37（11）：56-65. ［2］Shi Y, Zhao H, Shi Y, et al. Genome-wide association study identifies eight new risk loci for polycystic ovary syndrome ［J］. Nat Genet., 2012, 44(9): 1020-1025. ［3］Insenser M, Escobar-Morreale H F. Application of proteomics to the study of polycystic ovary syndrome ［J］. Journal of Endocrinological Investigation, 2011, 34(11):869-875. ［4］Skov V, Glintborg D, Knudsen S, et al.Pioglitazone enhances mitochondrial biogenesis and ribosomal protein biosynthesis in skeletal muscle in polycystic ovary syndrome ［J］.PLoS One. 2008,3(6):1142. ［5］Wood JR, Nelson-Degrave VL, Jansen E, et al. Valproate-induced alterations in human theca cell gene expression: clues to the association between valproate use and metabolic side effects ［J］. Physiol Genomics, 2005, 20(3):233-343. ［6］Cortón M, Botella-Carretero JI, Benguría A, et al.Differential gene expression profile in omental adipose tissue in women with polycystic ovary syndrome ［J］. J Clin Endocrinol Metab., 2007, 92(1):328-337. ［7］李威, 丛晶, 匡红影,等. 卵巢颗粒细胞基因表达异常与PCOS高雄激素血症 ［J］. 中华内分泌外科杂志, 2012,6(4):270-271. ［8］Jr O F, Matarese G, Di B S S, et al. Exon 6 and 2 peroxisome proliferator-activated receptor-gamma polymorphisms in polycystic ovary syndrome ［J］. Journal of Clinical Endocrinology & Metabolism, 2003, 88(12):5887-5892. ［9］夏燕, 王惠仔, 郑建淮. 慢性炎症因子在多囊卵巢综合征中的作用机制 ［J］. 中国实验动物学报, 2010, 18(5):414-416. ［10］陈慧玲,郑连文,路英丽,等. PCOS中低度慢性炎症相关因子的研究进展 ［J］. 中国妇幼保健,2016,31(9):2007-2011.

备注/Memo

备注/Memo:
广东省医学科学技术研究基金项目（项目编号：A2015455）
更新日期/Last Update: 2017-01-25