[1]张云鹤,李广太*.HER-2／neu和p16在外阴上皮内非瘤变中的 表达及其意义[J].中国计划生育和妇产科,2017,(1):64-68.
　ZHANG Yun-he,LI Guang-tai*.Expression of HER-2/neu and p16 protein in nonneoplastic epithelial disorders of vulva[J].Chinese Journal of Family Planning & Gynecotokology,2017,(1):64-68.
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HER-2／neu和p16在外阴上皮内非瘤变中的 表达及其意义
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2017年1期
页码:
64-68
栏目:
论著与临床
出版日期:
2017-01-25

文章信息/Info

Title:
Expression of HER-2/neu and p16 protein in nonneoplastic epithelial disorders of vulva
作者:
张云鹤; 李广太*
煤炭总医院妇产科
Author(s):
ZHANG Yun-he;  LI Guang-tai*
Department of Obstetrics and Gynecology，China Meitan General Hospital,Beijing 100028，P.R.China
关键词:
外阴上皮内非瘤变; 癌基因HER-2/neu; 抑癌基因p16
Keywords:
NNEDV; HER-2/neu; p16 protein expression
摘要:
目的针对外阴上皮内非瘤变（nonneoplastic epithelial disorders of vulva，NNEDV）难治疗易复发的特点，应用免疫组化方法检测外阴白色病变组织中人表皮生长因子受体-2 (human epidermal growth factor receptor 2，HER-2)／neu和p16蛋白表达水平的变化，并探讨其与外阴白色病变发生、发展的关系，以了解外阴白色病变的发病和治疗机制。方法选择煤炭总医院妇产科2014年5月至2016年1月NNEDV组织36份为研究组，其中硬化性苔癣(lichen sclerosus,LS)10例，鳞状上皮细胞增生(squamous hyperplasia，SH)18例，SH合并LS 8例;另选择阴道前后壁修补术者标本12份为正常对照组。应用免疫组化方法检测HER-2／neu和p16蛋白质的表达，并分析其与NNEDV发病的关系。结果发现p16表达率在正常外阴皮肤为25 %,高于外阴白色病变患者的111 %,差异无统计学意义（P＞005）。LS和SH组织中p16蛋白阳性表达率分别为10 %和56 %，差异无统计学意义(P>005)。正常外阴皮肤组织HER-2/neu蛋白表达率为83 %，外阴白色病变患者为555 %，明显高于正常外阴者（P＜005）。LS和SH组织中，HER-2/neu蛋白阳性表达率分别为40 ％和667 ％，两者比较差异有统计学意义(P<005)。结论原癌基因HER-2/neu和抑癌基因p16是调控细胞增殖周期进程的重要因子，其突变或缺失可能导致蛋白异常表达。它们参与了外阴异常增殖过程，在NNEDV甚至外阴上皮内瘤变及外阴鳞癌的发生、发展过程中起重要作用。
Abstract:
ObjectiveTo investigate the relationship between human epidermal grouth factor receptor2(HER-2)/neu and p16 protein and the expression of nonneoplastic epithelial disorders of vulva(NNEDV).In order to understand the pathogenesis and to seek effective treatments. MethodsBiopsies from 36 cases with NNEDV including 10 cases with lichen sclerosus(LS)，18 with squamous hyperplasia(SH) and 8 SH complicated with LS in China Meitan General Hospital from May 2014 to January 2016 were choosen as research group.They were examined for protein expression of HER-2/neu and p16 using immunohistochemical techniquesNormal vulvar tissues from 12 patients with other benign gynecologic diseases were used as controlResultsImmunopositive expression of p16 protein in the research group was 111％，with no significant difference from the control group(P＞005);the respective positive rate of p16 in LS and SH were 10% and 56% with no significant difference; 55.5％percent of patients with NNEDV were immunopositive for HER-2/neu，which was significantly higher than that of control group 83％(P＜005); there was significant difference between LS(40％) and SH patients(667％,P<005) in the expression of HER-2/neu proteinConclusionProto oncogene HER-2/neu and tumor suppressor gene p16 protein are important factors in regulating cell cycle progression. Their inactivation may play an important role in the genesis and development of NNEDV, vulvar intraepithelial neoplasia (VIN）and vulvar squamous cell carcinoma(VSCC).

参考文献/References:

［1］曹泽毅中华妇产科学［M］第3版北京：人民卫生出版社，2014：1255-1257 ［2］ChristopherpKR.妇产科诊断病理学［M］.北京:北京大学出版社，2007：109-143. ［3］HORN L C, BAUERFEIND U K, STRAUSS H G, et al. Vulval intestinal/enteric heterotopia with a 10-yr follow-up: a case report and review of the literature［J］. International Journal of Gynecological Pathology, 2014, 33(3): 258-262. ［4］MATOSO A, ROSS H M, CHEN S, et al. Squamous neoplasia of the scrotum:a series of 29 cases［J］. American Journal of Surgical Pathology, 2014, 38(7): 973-981. ［5］JHA A K, NIKBAKHT M, JAIN V, et al. p16(INK4a) and p15(INK4b) gene promoter methylation in cervical cancer patients［J］. Oncology Letters, 2012, 3(6): 1331-1335. ［6］LI Gang, JI Yuan, LIU Chen, et al. Reduced levels of p15INK4b, p16INK4a, p21cip1 and p27kip1 in pancreatic carcinoma［J］. Molecular Medicine Reports, 2012, 5(4): 1106-1110. ［7］马红梅，孙聚萍抑癌基因p16蛋白表达与外阴鳞状细胞癌关系的研究 ［J］中国肿瘤临床与康复，2013，20 （1）：6-8 ［8］Riethdorf S，Neffen EF，Cviko A，et alp16INKgA expression as biomarker for HPV 16-related vulvar neoplasias［J］.Human Pathlogy，2004，35(12)：1477-1483 ［9］Guerrero-Setas D，Pérez-Janices N，Ojer A，et al. Differential gene hypermethylation in genital lichen sclerosus and cancer：a comparative study ［J］.Histopathology，2013, 63(5): 659-669. ［10］Aide S，Lattario FR，Almeida G，et al. Promoter hypermethylation of death-associated protein kinase and p16 genes in vulvar lichen sclerosus ［J］.Low Genit Tract Dis，2012, 16(2): 133-139. ［11］Sahlberg KK，Hongisto V，Edgren H，et a1The HER2 amplieon includes several genes required for the growth and survival of HER2 positive breast cancer cells ［J］Molecular Oncology, 2013, 7(3): 392-401. ［12］Suzuki M，Shiraishi K，Yoshida A，et a1HER2 gene mutations in nonsmall cell lung carcinomas：Concurrence with her2 gene amplification and her2 protein expression and phosphorylation ［J］. Lung Cancer, 2015, 87(1): 14-22. ［13］张继刚，余春艳Annexin I、EGFR、C-erbB-2在外阴营养不良、外阴鳞癌中的表达 ［J］.西北国防医学杂志，2004，25(3)：197-199

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备注/Memo

备注/Memo:
首都临床特色应用研究（项目编号：Z121107001012033）
更新日期/Last Update: 2017-01-25