[1]李素廷,洪莉*,闵洁,等.电刺激对小鼠成纤维细胞L 929细胞活性、衰老及凋亡的影响[J].中国计划生育和妇产科,2017,(5):69-73.
　LI Su-ting,HONG Li*,MIN Jie,et al.Effects of electrical stimulation on activity, senescence and apoptosis of mouse fibroblast L929 cells[J].Chinese Journal of Family Planning & Gynecotokology,2017,(5):69-73.
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电刺激对小鼠成纤维细胞L 929细胞活性、衰老及凋亡的影响
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2017年5期
页码:
69-73
栏目:
论著与临床
出版日期:
2017-05-25

文章信息/Info

Title:
Effects of electrical stimulation on activity, senescence and apoptosis of mouse fibroblast L929 cells
作者:
李素廷; 洪莉*; 闵洁; 汤剑明; 胡鸣; 李洋; 刘耀丹; 李倩男; 何松明; 杨将
武汉大学人民医院妇产科
Author(s):
LI Su-ting;  HONG Li*;  MIN Jie;  TANG Jian-ming;  HU Ming;  LI Yang;  LIU Yao-dan;  LI Qian-nan;  HE Song-ming;  YANG Jiang
Department of Gynecology and Obstetrics, People’s Hospital of Wuhan University, Wuhan Hubei 430060, P.R.China
关键词:
小鼠成纤维细胞L 929细胞; 电刺激; 细胞活性; 衰老; 凋亡
Keywords:
mouse fibroblasts L929 cells;  electrical stimulation;  cell activity;  senescence;  apoptosis
分类号:
R 322
摘要:
目的观察不同强度及作用时长的电刺激对小鼠成纤维细胞L 929细胞活性、衰老及凋亡的影响。方法实验于2016年7~10月在武汉大学人民医院中心实验室进行。根据电刺激时长的不同，将小鼠成纤维细胞L 929细胞分为0 h组、2 h组、4 h组、6 h组，其中0 h组为对照组；根据电刺激强度的差异再将每组细胞分为50 mV/mm、100 mV/mm、200 mV/mm组，共计12组。采用CCK-8试剂盒、细胞衰老β－半乳糖苷酶染色试剂盒、线粒体膜电位检测试剂盒（JC-1）检测其活性、衰老及凋亡，每组实验重复4次。
Abstract:
ObjectiveTo observe the effects of different intensity and duration of electrical stimulation on the activity, senescence and apoptosis of mouse fibroblast L929 cells. MethodsThe experiment was conducted from July 2016 to October 2016 at the Central Laboratory of People’s Hospital of Wuhan University. The mouse fibroblast L929 cells were divided into 0 h group, 2 h group, 4 h group and 6 h group, and 0 h group was the control group according to the different duration of electrical stimulation. According to the difference of electrical stimulation intensity, each group of cells was divided into 50 mV / mm, 100 mV / mm, 200 mV / mm group, totaling 12 groups. The activity, senescence and apoptosis were detected by CCK-8 kit, cell senescence β-galactosidase staining kit and mitochondrial membrane potential detection kit (JC-1). Each group was repeated four times. ResultsCompared with the control group, cell viability was increased at 50 mV / mm 4 h ,6 h and 100 mV / mm 2 h group (P<005),while decreased at 50 mV / mm 2 h, 100 mV / mm 6 h, 200 mV / mm 2 h and 6 h group(P<005). 100 mV / mm 4 h and 200 mV / mm 4 h group of cell activity changes were not obvious. The cell senescence rate was decreased in 100 mV / mm 2 h group(P<005) while increased in 50 mV / mm 2 h, 4 h, 6 h, 100 mV / mm 6 h, 200 mV / mm 4 h and 6 h group(P<005). Cell senescence rate change was not obvious in 100 mV / mm 4 h and 200 mV / mm 2 h group. The proportion of apoptotic cells in 50 mV / mm 2 h and 100 mV / mm 2 h group decreased while increased in other groups. ConclusionThe activity, senescence and apoptosis status of mouse fibroblast L929 cells were different under different electric field intensity and duration. Therefore, while study the impact of electrical stimulation on cells,we should take full account of the strength of electrical stimulation and the duration of time. Among them, 100 mV / mm 2 h DC stimulation can improve the activity of mouse fibroblast L929 cells, inhibit its aging and apoptosis.

参考文献/References:

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更新日期/Last Update: 2017-05-25