[1]刘梦婷,杨潍潍,张翠娟,等.miR-125b在早发型重度子痫前期中对白细胞介素-8的调控研究[J].中国计划生育和妇产科,2017,(12):30-35.
　LIU Meng-ting,YANG Wei-wei,ZHANG Cui-juan,et al.Regulation of miR-125b on interleukin-8 in early-onsetsevere preeclampsia[J].Chinese Journal of Family Planning & Gynecotokology,2017,(12):30-35.
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miR-125b在早发型重度子痫前期中对白细胞介素-8的调控研究
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2017年12期
页码:
30-35
栏目:
论著与临床
出版日期:
2017-12-25

文章信息/Info

Title:
Regulation of miR-125b on interleukin-8 in early-onset severe preeclampsia
作者:
刘梦婷1; 杨潍潍2 ; 张翠娟3; 李清华4*; 李洁3*
潍坊医学院,1. 临床医学院;2.药学与生物科学学院细胞生物学教研室；3.附属医院产科；4. 公共卫生学院预防实验室
Author(s):
LIU Meng-ting1 ; YANG Wei-wei2; ZHANG Cui-juan3; LI Qing-hua4* ; LI Jie3*
1.College of Clinical Medicine;2.Department of Cell Biology,School of Pharmaceutical and Biological Science, Weifang Medical University， Weifang Shandong 261053,P.R.China;3.Department of Obstetrics, Affiliated Hospital of Weifang Medical University Weifan
关键词:
miR-125 b; EOSP; IL-8 ; SGPL 1; 滋养层
Keywords:
miR-125b;  EOSP; IL-8; SGPL1; trophoblast
分类号:
R 71424
摘要:
目的研究白细胞介素-8(interleukin-8, IL-8)在早发型重度子痫前期（early-onset severe pre-eclampsia，EOSP）患者中的表达水平及miR-125 b对其的调控作用。方法选取2014年1月至2015年7月在潍坊医学院附属医院及潍坊市妇幼保健院行剖宫产分娩且按照威廉姆斯产科学诊断为EOSP的产妇17例为EOSP组，随机选取同期无任何孕期并发症的正常产妇40例为对照组。通过定量聚合酶链反应检测EOSP患者及正常产妇胎盘中miR-125 b及IL-8的表达水平；用miR-125 b模拟物、miR-125 b抑制剂及其相应的空白对照分别转染人绒毛膜滋养层细胞株；荧光素酶试验进一步验证鞘氨醇-1-磷酸受体（sphingosine-1-phosphate lyase 1,SGPL 1）是否为miR-125 b的靶基因；用酶联免疫吸附试验确定细胞培养物上清液中的IL-8浓度。结果研究发现miR-125 b和IL-8在EOSP组患者的胎盘组织中失调，说明miRNA与IL-8在EOSP中有潜在联系。EOSP患者胎盘中miR-125 b 与SGPL1负相关，过表达miR-125 b可以显著降低SGPL 1的表达，且荧光素酶试验表明SGPL 1是miR-125 b的直接靶标；miR-125 b通过直接靶向SGPL 1来增加IL-8产生，并且这种效应可以被SGPL 1的过表达逆转。结论miR-125 b在EOSP的发展过程中调节IL-8，miR-125 b和SGPL 1可能成为EOSP潜在的临床预测和治疗的靶标。
Abstract:
ObjectiveTo investigate the expression of interleukin-8 (IL-8) in early-onset severe pre-eclampsia (EOSP) and its regulation by miR-125b.Methods57 pregnant women who had the cesarean section at Weifang Affiliated Hospital of Medical College and Weifang Maternal and Child Health Hospital from January 2014 to July 2015 were selected, and 17 of them diagnosed as EOSP according to Williams Obstetrics were selected as the EOSP group, the other 40 without any complications were selected as the control group. The control group were selected in random during the corresponding period. The levels of miR-125b and IL-8 in the placenta of EOSP patients and normal pregnant women were detected by quantitative polymerase chain reaction. Human chorionic trophoblast cell lines were transfected with miR-125b mimics, miR-125b inhibitor and its corresponding blank control, respectively. Luciferase assay further confirmed whether sphingosine-1-phosphate lyase 1 (SGPL 1) was the target gene of miR-125b; IL-8 concentrations in cell culture supernatants were determined by enzyme-linked immunosorbent assay(ELISA).ResultsThe study found that miR-125b and IL-8 in placental tissue disorder in EOSP patients, indicating that miRNA and IL-8 in EOSP were potentially linked. MiR-125b was negatively correlated with SGPL 1 in placenta of EOSP patients, and over-expression of miR-125b could significantly reduce SGPL 1 expression.Luciferase assay showed that SGPL 1 was the direct target of miR-125b. MiR-125b increased IL-8 production by targeting SGPL 1 directly, and this effect can be reversed by SGPL 1 overexpression. ConclusionMiR-125b regulates IL-8 during the development of EOSP, and miR-125b and SGPL 1 may be potential targets for clinical prediction and treatment of EOSP.

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备注/Memo

备注/Memo:
国家自然科学基金（项目编号：81601318）；山东省自然科学基金项目资助（项目编号：ZR2015HL021）
更新日期/Last Update: 2017-12-25