[1]刘佳,蔡小凤*.宫颈癌HSPC 238和RPS 27a基因检测的 临床意义研究[J].中国计划生育和妇产科,2018,(6):35-37,41.
　LIU Jia,CAI Xiao-feng*.Clinical significance of detection of HSPC238 and RPS27a genes in cervical cancer[J].Chinese Journal of Family Planning & Gynecotokology,2018,(6):35-37,41.
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宫颈癌HSPC 238和RPS 27a基因检测的 临床意义研究
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年6期
页码:
35-37,41
栏目:
论著与临床
出版日期:
2018-06-25

文章信息/Info

Title:
Clinical significance of detection of HSPC238 and RPS27a genes in cervical cancer
作者:
刘佳; 蔡小凤*
鄂东医疗集团黄石市中医医院妇产科
Author(s):
LIU Jia;  CAI Xiao-feng*
Department of Obstetrics and Gynecology, Huangshi Chinese Medicine Hospital, Huangshi Hubei 435000,P.R.China
关键词:
宫颈癌; HSPC 238基因; RPS 27a基因; 荧光定量PCR
Keywords:
uterine cervical cancer(UCC);  HSPC 238 gene;  RPS 27a gene;  realtime-PCR
分类号:
R 737.33
摘要:
目的检测HSPC 238和RPS 27a基因在宫颈癌中的表达并探讨其与宫颈癌的关系。方法选取2016年1月至2017年4月鄂东医疗集团黄石市中医医院手术治疗的宫颈癌患者92例，另取子宫良性病变切除的宫颈组织40例作为对照。荧光定量PCR检测HSPC 238和RPS 27a基因表达。结果宫颈癌组织HSPC 238基因的2-△Ct值为（0086±0014），显著低于对照组（0215±0032）（P<005）；宫颈癌组织RPS 27a基因的2-△Ct值为（0223±0037），显著高于对照组（0124±0013）（P<005）。HSPC 238基因2-△Ct值在FIGO Ⅲ、Ⅳ期为（0052±0007），显著低于Ⅰ、Ⅱ期（0122±0023）（P<005）；在有淋巴结转移组为（0068±0009），显著低于无淋巴结转移组（0102±0021）（P<005）；在中、低分化组为（0071±0010），显著低于高分化组（0106±0018）（P<005）。RPS 27a基因2-△Ct值在FIGO Ⅲ、Ⅳ期为（0253±0042），显著高于Ⅰ、Ⅱ期（0192±0027）（P<005）；在有淋巴结转移组为（0265±0044），显著高于无淋巴结转移组的（0184±0025）（P<005）；在中、低分化组为（0253±0041），显著低于高分化组的（0182±0024）（P<005）。宫颈癌患者HSPC 238基因表达与RPS 27a基因表达呈显著负相关（r=-0517，P=0026）。结论宫颈癌患者中HSPC 238基因低表达和RPS 27a基因高表达，HSPC 238基因表达减低和RP S27a基因表达升高与宫颈癌的临床分期、有无淋巴结转移以及肿瘤分化程度密切相关。
Abstract:
ObjectiveTo detect HSPC 238 and RPS 27a gene expression in uterine cervical cancer(UCC) and explore its clinical significance. Methods92 UCC patients in Huangshi Chinese Medidicine Hospital from Jan 2016 to April 2017 were enrolled in this study. Another 40 cases of benign uterine cervical tissues were used as control. HSPC 238 and RPS 27a gene expression was detected using realtime-PCR analysis. ResultsThe 2-△Ct of HSPC238 gene in UCC patients was (0086±0014), which was significantly lower than that of (0215±0032) in control group(P<05). The 2-△Ct of RPS 27a gene in UCC patients was (0223±0037), which was significantly higher than that of (0124±0013) in control group (P<005). The 2-△Ct of HSPC 238 gene in Ⅲ and Ⅳ stage group was (0052±0007), which was lower than that of (0122±0023) in Ⅰ and Ⅱ stage group(P<005). The 2-△Ct of HSPC 238 gene in lymph node metastasis group was (0068±0009), which was lower than that of (0102±0021) in non-lymph node metastasis group(P<005). The 2-△Ct of HSPC238 gene in median and low differentiation group was (0071±0010), which was lower than that of (0106±0018) in well differentiation group group(P<005). The 2-△Ct of RPS 27a gene in Ⅲ and Ⅳ stage group was (0253±0042), which was higher than that of (0192±0027) in Ⅰ and Ⅱ stage group(P<005). The 2-△Ct of RPS 27a gene in lymph node metastasis group was (0265±0044), which was higher than that of (0184±0025) in non-lymph node metastasis group(P<005). The 2-△Ct of RPS 27a gene in median and low differentiation group was (0253±0041), which was higher than that of (0182±0024) in well differentiation group group(P<005). The expression of HSPC 238 gene was negatively correlated with RPS 27a (r=-0517，P=0026) in patients with UCC. ConclusionLower-expression of HSPC 238 and higher-expression of RPS 27a was detected in UCC. Which was related to clinical stages, lymph node metastasis, and tumor differentiation.

参考文献/References:

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更新日期/Last Update: 2018-06-25