[1]贺昕红,蒋国庆*,李璇.DNA倍体分析在宫颈上皮内瘤变治疗后监测的 临床观察[J].中国计划生育和妇产科,2018,(8):19-21,29.
　HE Xin-hong,JIANG Guo-qing*,LI Xuan.The value of automatic DNA image cytometry in cervical intraepithelial neoplasia after the treatment[J].Chinese Journal of Family Planning & Gynecotokology,2018,(8):19-21,29.
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DNA倍体分析在宫颈上皮内瘤变治疗后监测的 临床观察
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年8期
页码:
19-21,29
栏目:
论著与临床
出版日期:
2018-08-25

文章信息/Info

Title:
The value of automatic DNA image cytometry in cervical intraepithelial neoplasia after the treatment
作者:
贺昕红1; 蒋国庆1*; 李璇2
1. 100050北京，首都医科大学附属北京友谊医院妇产科；2. 101149北京，首都医科大学附属北京潞河医院妇产科
Author(s):
HE Xin-hong1; JIANG Guo-qing1*; LI Xuan2
1.Department of Gynecology and Obstetrics,Beijing Friendship Hospital,Capital Medical University,100050 Beijing,P.R.China;2. Department of Gynecology and Obstetrics, Beijing Luhe Hospital, Capital Medical University,101149 Beijing,P.R.China
关键词:
DNA倍体分析; 宫颈上皮内瘤变; 人乳头瘤病毒
Keywords:
automatic DNA image cytometry; cervical intraepithelial neoplasia; human papillomavirus
分类号:
R 737.33
摘要:
目的探讨DNA倍体分析在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)治疗前后病情监测中的价值。方法选择首都医科大学附属北京友谊医院妇产科2015年4~12月经阴道镜活检病理诊断为CIN的患者84例及宫颈慢性炎症的患者30例，治疗前后进行随访观察。对纳入研究的患者手术治疗前及术后6个月复查时均行高危型人乳头瘤病毒（high-risk human papillomavirus, HR-HPV）、阴道镜活检及DNA倍体分析检测。结果① 随着宫颈病变程度增加，HR-HPV阳性率及DNA倍体检测阳性率增加，且N≥3个异倍体细胞数明显增加，差异有统计学意义(P<005)。HPV负荷量及宫颈上皮细胞DNA异倍体细胞数的变化趋势呈正相关，是递增趋势。② 治疗后半年复查HR-HPV、DNA倍体分析检测及阴道镜下宫颈组织活检，发现宫颈病变异常程度、HR-HPV及DNA倍体分析检测阳性率较前显著减少，差异有统计学意义(P<005)。③ 治疗前84例CIN患者中，有75例HR-HPV感染病例，其中57例伴有DNA倍体分析阳性。治疗后复查有10例HR-HPV持续感染病例，其中7例伴有DNA倍体分析阳性。结论① 随宫颈病变程度增加，DNA倍体检出率增加。且HPV负荷量及宫颈上皮细胞DNA异倍体细胞数的变化趋势呈正相关，是递增趋势。② 手术治疗能有效降低宫颈病变程度、HR-HPV阳性率及DNA倍体检测阳性率。③ 在HR-HPV持续感染的CIN患者中，同时行DNA倍体分析检测可辅助预测宫颈病变的进展情况。
Abstract:
ObjectiveTo investigate the monitoring value of DNA image cytometry in cervical intraepithelial neoplasia(CIN) before and after treatment. Methods84 patients confirmed as CIN, and 30 patients confirmed as chronic cervicitis by colposcopy and biopsy were chosen from Beijing Friendship Hospital,Capital Medical University in 2015 from April to December. These patients were examined with human papilloma virus(HPV) detection, multi-point biopsy under colposcopy and DNA image cytometry. Then we examined HPV, DNA image cytometry and colposcopy biopsy at the time of 6 months’ follow-up. Results① With the increase of cervical lesions, the positive rate of HR-HPV and the positive rate of DNA ploidy increased, and the number of N≥3 aneuploid cells increased significantly, the difference was statistically significant (P<005). The trend of HPV load and DNA aneuploid cell number in cervical epithelial cells was positively correlated and was an increasing trend;② After half a year of treatment, HR-HPV, DNA ploidy analysis and colposcopy biopsy were performed. The positive rate of cervical lesions, HR-HPV and DNA ploidy analysis were significantly lower than before. The difference was statistically significant(P<005).③ Of the 84 patients with CIN before treatment, 75 had HR-HPV infections, 57 of which were positive for DNA ploidy analysis. There were 10 cases of persistent HR-HPV infection after treatment, and 7 of them were positive for DNA ploidy analysis. Conclusion① As the degree of cervical lesions increases, the detection rate of DNA ploidy increases. Moreover, the trend of HPV load and the number of DNA aneuploid cells in cervical epithelial cells was positively correlated and was an increasing trend.② Surgical treatment can effectively reduce the degree of cervical lesions, HR-HPV positive rate and DNA ploidy detection positive rate.③ In patients with HR-HPV infected CIN, simultaneous DNA ploidy analysis can help predict the progression of cervical lesions.

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更新日期/Last Update: 2018-08-25