[1]徐锋,赵冰*,陈璐璐,等.与机械牵张盆底肛提肌卫星细胞间接共培养对大鼠骨髓 间充质干细胞成肌分化抗原和结蛋白表达的影响[J].中国计划生育和妇产科,2018,(3):44-49.
 XU Feng,ZHAO Bing*,CHEN Lu-lu,et al.Effect of mechanical stretch on the expressions of MyoD and Desmin in rat BMSCs with rat anus levator satellite cell co-culture[J].Chinese Journal of Family Planning & Gynecotokology,2018,(3):44-49.
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与机械牵张盆底肛提肌卫星细胞间接共培养对大鼠骨髓 间充质干细胞成肌分化抗原和结蛋白表达的影响
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年3期
页码:
44-49
栏目:
论著与临床
出版日期:
2018-03-25

文章信息/Info

Title:
Effect of mechanical stretch on the expressions of MyoD and Desmin in rat BMSCs with rat anus levator satellite cell co-culture
作者:
徐锋赵冰*陈璐璐 郑雪芹王肖帆王莹莹
郑州大学第三附属医院妇产科
Author(s):
XU FengZHAO Bing*CHEN Lu-lu ZHENG Xue-qinWANG Xiao-fanWANG Ying-ying
Department of Obstetrics and Gynecology,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou Henan 450052,P.R.China
关键词:
机械牵张盆底肛提肌卫星细胞骨髓间充质干细胞间接共培养MyoDDesmin
Keywords:
mechanical stretch rat anus levator satellite cell bone marrow mesenchymal stem cells indirect co-culture MyoD Desmin.
分类号:
R 7112
摘要:
目的研究与机械牵张的盆底肛提肌卫星细胞间接共培养对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成肌分化的影响;探索在体外条件下诱导BMSCs向肌细胞分化的微环境的创建以及成肌分化抗原(myogenic differentiation antigen,MyoD)和结蛋白(des minfilament,Desmin)蛋白的表达。方法选择7 d 龄SD大鼠,利用密度梯度离心法体外分离纯化大鼠BMSCs。取大鼠盆底肛提肌组织,自行分离传代,获得大鼠盆底肛提肌卫星细胞。对第3代盆底肛提肌卫星细胞施以10 %形变,1 Hz,12 h的周期性单向机械牵张刺激,机械牵张后的盆底肛提肌卫星细胞与纯化培养后的第4代BMSCs间接共培养6 d。采用实时定量RT-PCR方法检测共培养后BMSCs中MyoD和Desmin mRNA的表达情况;Western-blot法检测MyoD和Desmin的蛋白含量。结果与对照组相比,共培养组和机械牵张共培养组BMSCs 的MyoD和Desmin mRNA和蛋白的表达水平均有显著增高(P<005);与共培养组相比,机械牵张共培养组BMSCs 的MyoD和Desmin mRNA和蛋白的表达量也有显著增高(P<005)。结论与机械牵张刺激的盆底肛提肌卫星细胞间接共培养可以促进BMSCs合成MyoD和Desmin,诱导BMSCs向肌细胞分化。
Abstract:
ObjectiveThis study aims to evaluate the effect of mechanical stretch on the differentiation of bone marrow mesehchymal stem cells(BMSCs) with a co-culture with anus levator satellite cell, and the expressions of MyoD and Desmin. MethodsBMSCs were isolate

参考文献/References:

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备注/Memo

备注/Memo:
国家青年基金项目(项目编号:81300469 );河南省科技攻关计划(项目编号:132300410045)
更新日期/Last Update: 2018-03-25