[1]霍琰*,刘素新,李红艳,等.Vaspin、IL-6 在妊娠期糖尿病患者胎盘组织中的 表达[J].中国计划生育和妇产科,2018,(11):79-83.
　HUO Yan,LIU Su-xin,LI Hong-yan,et al.Expression of vaspin and IL-6 in placental tissues of patients with gestational diabetes mellitus[J].Chinese Journal of Family Planning & Gynecotokology,2018,(11):79-83.
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Vaspin、IL-6 在妊娠期糖尿病患者胎盘组织中的 表达
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年11期
页码:
79-83
栏目:
论著与临床
出版日期:
2018-11-25

文章信息/Info

Title:
Expression of vaspin and IL-6 in placental tissues of patients with gestational diabetes mellitus
作者:
霍琰1*;  刘素新1; 李红艳1;  李丽1; 冯静1 ; 宋光耀2
河北省人民医院，1.妇产科；2.内分泌科
Author(s):
HUO Yan1;  LIU Su-xin1;  LI Hong-yan1;  LI Li1;  FENG Jing1;  SONG Guang-yao2
1.Department of Obstetrics and Gynecology; 2.Department of Endocrinology, People’s Hospital of Hebei Province，Shijiazhuang Hebei 050051，P.R.China
关键词:
妊娠期糖尿病; 内脏脂肪组织来源的丝氨酸蛋白酶抑制剂（vaspin）; BeWo细胞; 白细胞介素-6
Keywords:
gestational diabetes mellitus; vaspin; BeWo cells; IL-6
分类号:
R 714.256
摘要:
目的测定妊娠期糖尿病(gestational diabetes mellitus，GDM)及正常妊娠妇女胎盘组织中丝氨酸蛋白酶抑制剂（vaspin）及白细胞介素-6（interleukin-6, IL-6）的表达，观察IL-6对BeWo细胞vaspin表达的影响，探讨vaspin、IL-6在GDM中的作用及相互关系。方法选择2012年10月至2013年10月于河北省人民医院行择期剖宫产术57例孕妇，根据孕妇有无GDM分为GDM组（30例）和正常妊娠（normal glucose tolerance，NGT）组（27例）。取两组孕妇胎盘组织，实时荧光定量反转录PCR测定胎盘组织vaspin、IL-6 mRNA表达水平，免疫蛋白印迹法检测胎盘组织vaspin及IL-6蛋白的表达水平；用不同浓度的IL-6分别与BeWo细胞共培养，留取细胞，测定BeWo细胞vaspin mRNA和蛋白表达水平。结果GDM组胎盘组织vaspin mRNA及蛋白表达水平与NGT组比较［(060±032) vs (068±032); (030±008 ) vs (039±026 )］，差异无统计学意义（P＞005）；IL-6 mRNA及蛋白表达水平高于NGT组［(135±067 ) vs (082±047 )；(123±025) vs (054±020)］（P<005）；GDM组胎盘组织vaspin mRNA和蛋白表达与新生儿体质量呈负相关（r=-059、-088, P＝001、<001）。不同浓度的IL-6均可抑制BeWo细胞vaspin mRNA及蛋白表达。结论vaspin与GDM的发生有关，并参与胎儿生长发育；IL-6可能参与了vaspin的调控。
Abstract:
ObjectiveTo investigate the mRNA and protein expression of vaspin and interleukin-6(IL-6) in the placenta tissues in pregnant women with or without gestational diabetes mellitus(GDM)，and to observe the changes of the vaspin expression on BeWo cells after cultured with different concentrations of IL-6, analyzing their correlationship and roles in GDM．MethodsA total of 57 patients who underwent elective cesarean section in People’s Hospital of Hebei Province from October 2012 to October 2013 were selected and divided into GDM group and normal glucose tolerance (NGT) group. The placental tissues of the two groups were taken. The expression levels of vaspin and IL-6 mRNA in placenta were detected by real-time fluorescent quantitative reverse transcription-PCR. The expression levels of vaspin and IL-6 protein in placenta were detected by immunoblotting. Different concentrations of IL-6 were used to co-culture with BeWo cells, and determine the expression level of vaspin mRNA and protein in BeWo cells. ResultsThe expression of vaspin mRNA and protein in placenta of GDM group was compared with those of NGT group ［(060±032) vs (068±032); (030±008) vs (039±026)］, the differences were not statistically significant (P>005). The expression levels of IL-6 mRNA and protein were higher than those in the NGT group ［(135±067) vs (082±047); (123±025) vs (054±020)］ (P<005). The expression of vaspin mRNA and protein in placenta of GDM group was negatively correlated with the body weight of newborns (r=-059, -088；P=001，<001). Different concentrations of IL-6 can inhibit the expression of vaspin mRNA and protein in BeWo cells. Conclusion Vaspin is involved in the development of GDM and participates in fetal growth and development; IL-6 may be involved in the regulation of vaspin.

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更新日期/Last Update: 2018-11-25