[1]陈丹玲,刘国成*,梁海英,等.miRNA-206和IGF-1在早发型子痫前期孕妇 血清中的表达水平及临床意义[J].中国计划生育和妇产科,2018,(6):79-83,91.
　CHEN Dan-ling,LIU Guo-cheng*,LIANG Hai-ying,et al.Expression levels and clinical significances of miRNA-206 and IGF-1 in the serum of pregnant women with early onset preeclampsia[J].Chinese Journal of Family Planning & Gynecotokology,2018,(6):79-83,91.
点击复制

miRNA-206和IGF-1在早发型子痫前期孕妇 血清中的表达水平及临床意义
分享到：

《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年6期
页码:
79-83,91
栏目:
论著与临床
出版日期:
2018-06-25

文章信息/Info

Title:
Expression levels and clinical significances of miRNA-206 and IGF-1 in the serum of pregnant women with early onset preeclampsia
作者:
陈丹玲; 刘国成*; 梁海英; 余晖; 彭苏珺
广东省妇幼保健院产科
Author(s):
CHEN Dan-ling; LIU Guo-cheng*; LIANG Hai-ying; YU Hui; PENG Su-jun
Department of Obstetrics,Maternal and Child Health Hospital of Guangdong Province， Guangzhou Guangdong 511400,P.R.China
关键词:
早发型子痫前期; 血清; microRNA-206; 胰岛素样生长因子-1
Keywords:
early onset preeclampsia（EOPE）; serum; microRNA-206;  insulin-like growth factors-1（IGF-1）
分类号:
R 71424+5
摘要:
目的探究microRNA-206（miRNA-206）和胰岛素样生长因子-1（insulin-like growth factor-1，IGF-1）在早发型子痫前期（early onset preeclampsia，EOPE）孕妇血清中的表达水平及临床意义。方法选择2016年1月至2017年2月广东省妇幼保健院收治的105例孕妇为研究对象，包括35例正常晚期妊娠孕妇（正常妊娠组）、35例轻度EOPE孕妇（轻度EOPE组）和35例重度EOPE孕妇（重度EOPE组）。所有孕妇均在入院次日清晨空腹状态下采集肘静脉血，实时定量PCR（qRT-PCR）技术检测各组血清miRNA-206水平，酶联免疫（ELISA）检测各组血清IGF-1水平；利用受试者工作特征（ROC）曲线分析血清miRNA-206与IGF-1水平对EOPE的诊断效能。结果① 轻度、重度EOPE组血清miRNA-206水平均高于正常妊娠组（t=2689、5404，P=0009、0000），重度EOPE组高于轻度EOPE组（t=2139，P=0036）。② 轻度、重度EOPE组血清IGF-1水平均低于正常妊娠组（t=2853、5136，P=0006、0000），重度EOPE组低于轻度EOPE组（t=2201，P=0031）。③ EOPE患者血清miRNA-206水平与IGF-1水平呈负相关关系（r=-0364，P=0002）。④ ROC曲线分析结果发现，血清miRNA-206诊断EOPE的曲线下面积为0749，其诊断的敏感度为6768 %、特异度为7372 %；血清IGF-1诊断EOPE的曲线下面积为0668，其诊断的敏感度为4002 %、特异度为9677 %。结论EOPE患者miRNA-206表达水平较健康孕妇高、IGF-1血清表达水平较健康孕妇低，提示监测血清miRNA-206、IGF-1水平对早期诊断EOPE具有一定的参考价值。
Abstract:
ObjectiveTo explore the expression levels and clinical significances of microRNA-206 (miRNA-206) and insulin-like growth factor-1 (IGF-1) in the serum of pregnant women with early onset preeclampsia (EOPE).MethodsA total of 105 pregnant women in Maternal and Child Health Hospital of Guangdong Province from January 2016 to February 2017 were selected, including 35 cases of normal late pregnant women, 35 cases of mild EOPE pregnant women and 35 cases of severe EOPE pregnant women. All pregnant women were collected the venous blood in the morning of the next day of admission to the hospital, and measured the level of miRNA-206 in each group by real-time quantitative PCR (qRT-PCR); the serum levels of IGF-1 were detected by enzyme linked immunosorbent assay (ELISA); the diagnostic efficacy of serum miRNA-206 and IGF-1 levels on EOPE was analyzed by the ROC curve. Results① The level of serum miRNA-206 in the mild EOPE group and severe EOPE group was higher than that in the normal late pregnancy group (t=2689、5404, P=0009、0000）, the severe EOPE group was higher than that the mild EOPE group (t=2139, P=0036).② The level of serum IGF-1 in the mild EOPE group and severe EOPE group was lower than that in the normal late pregnancy group (t=2853、5136, P=0006、0000）,the level of serum IGF-1 in the severe EOPE group was lower than that in the mild EOPE group (t=2201, P=0031).③ The level of serum miRNA-206 was negatively correlated with the level of IGF-1 in patients with EOPE (r=-0364, P=0002).④ The results of ROC curve analysis showed that, the area under the curve of serum miRNA-206 for diagnosis of EOPE was 0749, the sensitivity of the diagnosis was 6768 %, and the specificity was 7372 %; the area under the curve of serum IGF-1 for diagnosis of EOPE was 0668, and the sensitivity of the diagnosis was 4002 %, and the specificity was 9677 %. ConclusionThe expression level of miRNA-206 was higher than that of healthy pregnant women and IGF-1 lower than that of healthy pregnant women, it is suggested that the monitoring of serum miRNA-206 and IGF-1 levels has a certain reference value for early diagnosis of EOPE.

参考文献/References:

［1］魏璐，刘兴会，陈锰．子痫前期风险预警的研究进展 ［J］．中国妇幼保健，2017，32（20）：5173-5177． ［2］倪琰琰，程蔚蔚．早发型子痫前期发病机制的研究进展 ［J］．山东医药，2016，56（8）：101-104． ［3］Akehurst C, Small HY, Sharafetdinova L, et al. Differential expression of microRNA-206 and its target genes in preeclampsia［J］. J Hypertens, 2015, 33(10): 2068-2074. ［4］Panjeta P, Ghalaut V S, Bala J, et al. Inverse correlation between insulin-like growth factor-1 and leptin levels in preeclampsia［J］.Journal of Basic and Clinical Reproductive Sciences，2016, 5(2):94-99. ［5］Dubova EA, Pavlov KA, Lyapin VM, et al. Expression of insulin-like growth factors in the placenta in preeclampsia ［J］. Bull Exp Biol Med, 2014, 157(1): 103-107. ［6］乐杰．妇产科学 ［M］．第7版.北京：人民卫生出版社，2008． ［7］Phillips C, Boyd M. Assessment, management, and health implications of early-onset preeclampsia［J］. Nurs Womens Health, 2016, 20(4): 400-414. ［8］黄婷．早发型和晚发型重度子痫前期临床指标的对比研究 ［J］．中国计划生育和妇产科，2017，9（8）：27-30． ［9］Veerbeek JH, Hermes W, Breimer AY, et al. Cardiovascular disease risk factors after early-onset preeclampsia, late-onset preeclampsia, and pregnancy-induced hypertension［J］. Hypertension, 2015, 65(3): 600-606. ［10］ZHOU Jianqing, SHAO Guofeng, CHEN Xiaoliang, et al. MiRNA 206 and miRNA 574-5p are highly expression in coronary artery disease ［J］. Biosci Rep, 2015, 36(1): e00295. ［11］WU Heng, ZHANG Tianpeng, PAN Fei, et al. MicroRNA-206 prevents hepatosteatosis and hyperglycemia by facilitating insulin signaling and impairing lipogenesis ［J］. J Hepatol, 2017, 66(4): 816-824. ［12］REN X-l, HE G-y, LI X-m, et al. MicroRNA-206 functions as a tumor suppressor in colorectal cancer by targeting FMNL2 ［J］. J Cancer Res Clin Oncol, 2016, 142(3): 581-592. ［13］JIN Peng, GU Wenzhu, LAI Yayu, et al. The circulating MicroRNA-206 level predicts the severity of pulmonary hypertension in patients with left heart diseases ［J］. Cell Physiol Biochem, 2017, 41(6): 2150-2160. ［14］TANG Xinyi, TIAN Xinyu, ZHANG Yue, et al. Correlation between the frequency of Th17 cell and the expression of microRNA-206 in patients with dermatomyositis ［J］. Clin Dev Immunol, 2013(6): 345347. ［15］Cornelius DC, Amaral LM, Wallace K, et al. Reduced uterine perfusion pressure T-helper 17 cells cause pathophysiology associated with preeclampsia during pregnancy ［J］. Am J Physiol Regul Integr Comp Physiol, 2016, 311(6): R1192-R1199. ［16］Barnie PA, XIN L, Yueqin L, et al. IL-17 producing innate lymphoid cells 3(ILC3)but not Th17 cells might be the potential danger factor for preeclampsia and other pregnancy associated diseases［J］. International Journal of Clinical & Experimental Pathology, 2015, 8(9): 11100-11107. ［17］刘震忠．血清及胎盘组织中IGF-1及NO的表达与早发型子痫前期相关性分析 ［J］．中国妇幼保健，2017，32（12）：2602-2604． ［18］LIU K, WU H-y, XU Y-h. Study on the relationship between the expression of IGF-1 in umbilical cord blood and abnormal glucose metabolism during pregnancy ［J］. Eur Rev Med Pharmacol Sci, 2017, 21(4): 647-651. ［19］王斌，白睿．血清炎性因子及胰岛素样生长因子相关指标与妊娠期高血压疾病的相关性研究 ［J］．海南医学院学报，2017，23（5）：698-700． ［20］LIAO Shutan, Vickers MH, Taylor RS, et al. Maternal serum IGF-1, IGFBP-1 and 3, and placental growth hormone at 20 weeks' gestation in pregnancies complicated by preeclampsia［J］. Pregnancy Hypertens, 2017, 7 (10): 149-154.

相似文献/References:

[1]孙桢,黄立,夏勇,等.妊娠期糖尿病与核转录因子NF-κBp65相关性研究[J].中国计划生育和妇产科,2015,(04):0.
　SUN Zhen,HUANG Li,XIA Yong,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2015,(6):0.
[2]李亚梅,杨星宇,赵雪娅,等.早发型和晚发型子痫前期自噬与凋亡水平差异 及相互关系研究[J].中国计划生育和妇产科,2019,(11):38.
　LI Ya-mei,YANG Xing-yu,ZHAO Xue-ya,et al.The differences and interrelation of trophoblastic autophagy and apoptosis between early and late-onset preeclampsia[J].Chinese Journal of Family Planning & Gynecotokology,2019,(6):38.
[3]王德敏*,吴桂梅,李冀云,等.子痫前期患者血清中miR200 c的表达 及其临床意义[J].中国计划生育和妇产科,2020,(4):54.
　WANG De min*,WU Gui mei,LI Ji yun,et al.Expression of miR200 c in serum of patients with preeclampsia and its clinical significance[J].Chinese Journal of Family Planning & Gynecotokology,2020,(6):54.
[4]赵嘉欣,汤小晗,卢美松*.氧化应激标志物与子宫内膜异位症的研究进展[J].中国计划生育和妇产科,2020,(9):5.

更新日期/Last Update: 2018-06-25