[1]何金英,任宇,刘慧,等.纤维蛋白基因对大鼠早期胚胎发育的影响[J].中国计划生育和妇产科,2019,(11):81-84.
 HE Jin-ying,REN Yu,LIU Hui,et al.Effect of FBL gene on early embryo development in rats[J].Chinese Journal of Family Planning & Gynecotokology,2019,(11):81-84.
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纤维蛋白基因对大鼠早期胚胎发育的影响
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2019年11期
页码:
81-84
栏目:
论著与临床
出版日期:
2019-11-25

文章信息/Info

Title:
Effect of FBL gene on early embryo development in rats
作者:
何金英任宇刘慧黄翔华马玉珍*
内蒙古自治区人民医院生殖中心
Author(s):
HE Jin-ying REN YuLIU HuiHUANG Xiang-huaMA Yu-zhen*
Department of Reproductive Medicine Center,Inner Mongolia People's Hospital, Hohhot Inner Mongolia Autonomous Region 010017,P.R.China
关键词:
Fibrillarin基因早期胚胎发育囊胚
Keywords:
fibrillarin gene early embryo development blastocyst
分类号:
R 71425;R 654.3
摘要:
目的研究纤维蛋白(fibrillarin,FBL)基因对大鼠早期胚胎发育的影响。 方法选取健康、性成熟的大鼠30只,按照1:1的比例将雌鼠放置于雄鼠笼子里,同笼交配。采集大鼠胚胎,构建FBL上调、下调慢病毒载体,免疫荧光技术检测FBL在胚胎细胞中的表达,使用荧光定量RT-PCR技术检测FBL mRNA、Oct 4、Sox 2、FGF 4表达量,并作组间比较。结果FBL在卵裂期胚胎中特异性染色不明显,而在囊胚中可被检测到有表达。上调组大鼠囊胚期FBL mRNA表达量、FBL表达量高于对照组,8-细胞阶段卵裂
Abstract:
ObjectiveTo study the effect of fibrillarin (FBL) gene on early embryo development in rats. MethodsThirty healthy and sexually mature rats were selected and placed in male cages at a ratio of 1∶1 and mated with cages. The rat embryos were collected, FBL

参考文献/References:

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备注/Memo

备注/Memo:
内蒙古自治区人民医院院内基金项目(项目编号:2016071)
更新日期/Last Update: 2019-11-25