[1]王德敏*,吴桂梅,李冀云,等.子痫前期患者血清中miR200 c的表达 及其临床意义[J].中国计划生育和妇产科,2020,(4):54-58.
　WANG De min*,WU Gui mei,LI Ji yun,et al.Expression of miR200 c in serum of patients with preeclampsia and its clinical significance[J].Chinese Journal of Family Planning & Gynecotokology,2020,(4):54-58.
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子痫前期患者血清中miR200 c的表达 及其临床意义
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2020年4期
页码:
54-58
栏目:
论著与临床
出版日期:
2020-04-25

文章信息/Info

Title:
Expression of miR200 c in serum of patients with preeclampsia and its clinical significance
作者:
王德敏*; 吴桂梅; 李冀云; 金燕; 邢晓红; 曹艳敏
沧州市中心医院产科
Author(s):
WANG Demin*; WU Guimei; LI Jiyun; JIN Yan; XING Xiaohong; CAO Yanmin
Department of Obstetrics, Cangzhou Central Hospital, Cangzhou Hebei 061000，P.R.China
关键词:
子痫前期; miR200 c; 血清; 诊断价值; 妊娠结局
Keywords:
preeclampsia;  miR200 c;  serum;  diagnostic value;  pregnancy outcomes
分类号:
R 71424
摘要:
目的 探讨子痫前期（preeclampsia，PE）患者miR200 c的表达及其临床意义。方法 选取2017年1月到2019年2月在沧州市中心医院治疗的PE患者125例，其中72例轻度PE患者纳入轻度PE组，53例重度PE患者纳入重度PE组。另选取同期产检的健康孕妇50例为对照组。采用实时荧光定量PCR检测3组患者血清miR200 c的相对表达量，根据PE患者血清miR200 c的表达情况分为miR200 c高表达组和miR200 c低表达组。比较3组研究对象一般资料和血清miR200 c水平，以及不同miR200 c水平PE患者的一般资料和不良妊娠结局，分析血清miR200 c对PE和重度PE的诊断价值。结果 重度PE组、轻度PE组、对照组的收缩压、舒张压、24 h尿蛋白定量、miR200 c水平逐次降低，差异有统计学意义（P<005），miR200 c高表达组的收缩压、舒张压、24 h尿蛋白定量水平、羊水污染≥Ⅲ度、胎儿宫内窘迫、子痫的发生率均高于miR200 c低表达组，差异有统计学意义（P<005），PE患者血清miR200 c的相对表达量与收缩压、舒张压、24 h尿蛋白定量呈正相关（P<005），经ROC分析，血清miR200 c对PE的诊断价值较高，曲线下面积为0894（95 %CI：08490940），对重度PE亦有较高诊断价值，曲线下面积为0816（95 %CI：07410892）。结论 PE患者血清miR200 c的表达水平高于正常孕妇，其表达水平可能与患者的病情严重程度和妊娠结局有关，血清miR200c对PE和重度PE均有较高诊断价值。
Abstract:
ObjectiveTo investigate the expression and clinical significance of microRNA200 c (miR200 c) in serum of patients with preeclampsia（PE）.Methods125 patients with PE who treated in Cangzhou Central Hospital from January 2017 to February 2019 were enrolled. In which 72 patients with mild PE were included in the mild PE group, and 53 patients with severe PE were included in the severe PE group. Another 50 healthy pregnant women who were diagnosed in our hospital during the same period were selected as the control group. Realtime quantitative PCR was used to detect the relative expression of serum miR200 c in the three groups, and PE patients were divided into miR200 c high expression group and miR200 c low expression group according to the expression of serum miR200 c. The general data and serum miR200 c levels of the three groups of subjects, as well as the general data and adverse pregnancy outcomes of PE patients with different miR200 c levels were compared. The diagnostic value of serum miR200 c for PE and severe PE was analyzed. ResultsSystolic blood pressure, diastolic blood pressure, 24 h urine protein quantitation and miR200 c levels were significantly decreased in severe PE group, mild PE group and control group, the difference was statistically significant (P<005).The miR200 c high expression group had higher systolic blood pressure, diastolic blood pressure, 24 h urine protein quantitative, and higher incidence rate of amniotic fluid pollution ≥ III degree, fetal intrauterine distress, and eclampsia than miR200 c low expression group,the difference was statistically significant (P<005). The relative expression of serum miR200 c in PE patients was positively correlated with systolic blood pressure, diastolic blood pressure and 24 h urine protein (P<005). ROC analysis showed that serum miR200 c had a high diagnostic value for PE,the area under the curve was 0894 (95 % CI: 08490940), which also had a high diagnostic value for severe PE,the area under the curve was 0816 (95 % CI：07410892). ConclusionThe level of serum microRNA200 c in preeclampsia patients is higher than that in normal pregnant women, and the expression level may be related to the severity of the disease and pregnancy outcomes. Serum microRNA200 c has high diagnostic value for PE and severe PE.

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备注/Memo

备注/Memo:
沧州市科学计划自筹经费项目（项目编号：183302044）
更新日期/Last Update: 2020-04-25