[1]华人意,程蔚蔚*.人羊膜细胞中趋化因子的表达及调节机制[J].中国计划生育和妇产科,2020,(6):51-54.
　HUA Renyi,CHENG Weiwei*.Expression and regulation mechanism of chemokine in human amniotic cells[J].Chinese Journal of Family Planning & Gynecotokology,2020,(6):51-54.
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人羊膜细胞中趋化因子的表达及调节机制
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2020年6期
页码:
51-54
栏目:
论著与临床
出版日期:
2020-06-25

文章信息/Info

Title:
Expression and regulation mechanism of chemokine in human amniotic cells
作者:
华人意;  程蔚蔚*
上海交通大学附属国际和平妇幼保健院产前诊断中心
Author(s):
HUA Renyi; CHENG Weiwei*
Prenatal Diagnosis Center,International Peace Maternity and Child Health Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200030,P.R.China
关键词:
早产; 趋化因子; 羊膜细胞
Keywords:
preterm labor; chemokines; amnion
分类号:
R 392.12
摘要:
目的通过体外模拟分娩时的炎症内环境，了解羊膜细胞在分娩启动中发挥的作用。方法随机选取2018年1~6月中国福利会国际和平妇幼保健院产科足月择期剖宫产病例，分为研究组和对照组，每组6例。取新鲜完整胎盘，分离羊膜细胞进行细胞培养。按分组加入白介素1β（interleukin 1 beta，IL1β）、肿瘤坏死因子α（tumor necrosis factor alpha，TNFα ）、前列腺素E 2 （prostaglandin E 2，PGE 2）及前列腺素F 2α（prostaglandin F 2 alpha ，PGF 2α），于1 h、6 h和24 h收获细胞。检测羊膜细胞中各趋化因子的表达。结果在IL1β /TNFα的作用下，羊膜细胞中趋化因子CCL 2，CCL 5，CCL 20，CXCL 1，CXCL 5和CXCL 8表达增加，而在前列腺素作用下趋化因子CCL 2，CCL 5，CXCL 1，CXCL 8表达下调，CCL 20表达增加。结论本研究展现了分娩过程中羊膜细胞中的免疫调节变化，羊膜细胞通过分泌趋化因子诱导分娩炎症反应，可能早于子宫肌中的炎症反应。本研究为了解分娩启动机制提供了依据。
Abstract:
ObjectiveBy simulating the inflammatory internal environment during labor in vitro, we can understand the role of amnion cells in labor initiation. MethodsRandomly selected cases of full-term cesarean section from the Obstetrics Department of International Peace Maternity and Child Health Hospital of China Welfare Society from January to June 2018 were divided into a research group and a control group,each with 6 cases.Take fresh intact placenta, isolate amniotic membrane cells for cell culture. Add interleukin 1β (interleukin 1 beta, IL1β), tumor necrosis factor alpha (tumor necrosis factor alpha, TNFα), prostaglandin E 2 (prostaglandin E 2, PGE 2) and prostaglandin F 2α (prostaglandin F 2 alpha, PGF 2α). Cells were harvested at 1 h, 6 h and 24 h. Detect the expression of various chemokines in amniotic cells.ResultsUnder the action of IL1β / TNFα, the expression of chemokines CCL 2, CCL 5, CCL 20, CXCL 1, CXCL 5 and CXCL 8 increased in amniotic cells, while the expression of chemokines CCL 2, CCL 5, CXCL 1 and CXCL 8 under the action of prostaglandins downregulated, CCL 20 expression increased.ConclusionThis study demonstrates the changes in immune regulation in amniotic cells during labor. Amniotic cells secrete chemokines to induce inflammatory responses during labor, which may be earlier than those in uterine muscle. This study provides a basis for understanding the mechanism of labor delivery.

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更新日期/Last Update: 2020-06-25