[1]韩翠香.长链非编码RNA MALAT1在子宫内膜癌中的表达及临床意义[J].中国计划生育和妇产科,2016,(6):51-55.
　HAN Cui-xiang.The expression and clinical significance of long non-coding RNA MALAT1 in endometrial cancer[J].Chinese Journal of Family Planning & Gynecotokology,2016,(6):51-55.
点击复制

长链非编码RNA MALAT1在子宫内膜癌中的表达及临床意义
分享到：

《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2016年6期
页码:
51-55
栏目:
论著与临床
出版日期:
2016-06-25

文章信息/Info

Title:
The expression and clinical significance of long non-coding RNA MALAT1 in endometrial cancer
作者:
韩翠香
陕西榆林神木县医院妇产科
Author(s):
HAN Cui-xiang
Department of Obstetrics and Gynecology, Shenmu county Hospital，Yulin Shaanxi 719300，P.R.China
关键词:
MALAT1; 子宫内膜癌; ROC曲线; 临床病理特征; 生物标记物
Keywords:
MALAT1;  endometrial cancer;  ROC curve;  clinicopathological feature;  biomarker
分类号:
R 711.74
摘要:
目的探讨子宫内膜癌组织中长链非编码RNA MALAT1的表达及临床意义。方法采用实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction ，qRT-PCR）方法检测榆林市神木县医院2009年9月至2010年9月确诊的104例子宫内膜癌患者新鲜子宫内膜癌组织标本及其对应的癌旁2 cm组织标本中MALAT1的表达，分析其与子宫内膜癌的临床病理特征关系以及诊断价值和预后关系。结果MALAT1在子宫内膜癌组织中的表达明显高于子宫内膜癌旁组织(P< 005)；MALAT1在子宫内膜癌高、中分化组和低分化组中的阳性表达差异无统计学意义（P> 005）；MALAT1在子宫内膜癌组织中的表达与淋巴结转移、肿瘤浸润深度以及血管侵袭相关，差异均有统计学意义（P＜005）；ROC曲线AUC=07314（95 %CI，06141~08487；P＜005）；MALAT1高表达和低表达组在总生存时间 OS（χ2=7269，P＜005）及无进展生存时间PFS（χ2=5182，P＜005）上的差异有统计学意义。 结论长链非编码RNA MALAT1 在子宫内膜癌中是高表达的，可以作为子宫内膜癌的新型生物标记物和诊断靶标。
Abstract:
ObjectiveTo investigate the expression and clinical significance of long non-coding RNA （lncRNA） metastasis-associated lung adenocarcinoma transcript 1(MALAT1) in endometrial cancer. Methods104 endometrial cancer tissue samples and 104 corresponding adjacent tissues were collected. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect MALAT1 mRNA expression and its association with patient clinicopathological characteristics and overall survival of patients was further analyzed. A receiver operating characteristic (ROC) curve was constructed to determine the diagnostic value of MALAT1. ResultsMALAT1 expression was significantly upregulated in the endometrial cancer tissues compared with the matched adjacent tissues(P<005); MALAT1 expression was no significant different in tumor differentiation (P＞005)；There was significant differences between lymph node metastasis, tumor infiltration level, vascular invasion in term of MALAT1 expression; the area under the ROC curve was 07314(95 %CI,06141~08487;P＜005)；The high MALAT1 expression and low expression groups are statistically significant in overall survival time (χ2=7269，P＜005) and progression-free survival time (χ2=5182，P＜005). ConclusionLncRNA MALAT1 may be involved in carcinogenesis and may prove useful as a biomarker for diagnosis and prognostic significance in patients with endometrial cancer.

参考文献/References:

［1］ESTELLER M. Non-coding RNAs in human disease ［J］. Nature Reviews Genetics, 2011, 12(12): 861-874. ［2］Yoshimoto R. MALAT1 long non-coding RNA in cancer ［Z］, 2015. ［3］Gutschner T, Hmmerle M, Diederichs S. MALAT1—a paradigm for long noncoding RNA function in cancer ［J］. Journal of Molecular Medicine (Berlin, Germany), 2013, 91(7): 791-801. ［4］SIEGEL R, MA Jie-min, ZOU Zhao-hui, et al. Cancer statistics ［J］. CA-A Cancer Journal for Clinicians, 2014, 64(1): 9-29. ［5］GUTSCHNER T, DIEDERICHS S. The hallmarks of cancer: a long non-coding RNA point of view ［J］. RNA Biology, 2012, 9(6): 703-719. ［6］Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs:insights into functions ［J］. Nature Reviews Genetics, 2009, 10(3): 155-159. ［7］YANG Lin, ZHANG Jie, JIANG Anli, et al. Expression profile of long non-coding RNAs is altered in endometrial cancer ［J］. International Journal of Clinical and Experimental Medicine, 2015, 8(4): 5010-5021. ［8］HE Xiaoying, BAO Wei, LI Xiaocui, et al. The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis ［J］. International Journal of Molecular Medicine, 2014, 33(2): 325-332. ［9］YOU Q-y,h tao, LING B. Long noncoding RNA HOX transcript antisense intergenic RNA(HOTAIR)as a foe and novel potential therapeutic target for endometrial carcinoma ［J］. International Journal of Gynecological Cancer, 2014, 24(9): 1536. ［10］GUO Chen, SONG Wei-qi, SUN Ping, et al. LncRNA-GAS5 induces PTEN expression through inhibiting miR-103 in endometrial cancer cells ［J］. Journal of Biomedical Science, 2015, 22(1): 213-214. ［11］JI Q, ZHANG L, LIU X, et al. Long non-coding RNA MALAT1 promotes tumour growth and metastasis in colorectal cancer through binding to SFPQ and releasing oncogene PTBP2 from SFPQ/PTBP2 complex ［J］. British Journal of Cancer, 2014, 111(4): 736-748. ［12］WU Xiang-song, WANG Xu-an, WU W G, et al. MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway ［J］. Cancer Biology & Therapy, 2014, 15(6): 806-814. ［13］FAN Yu, SHEN Bing, TAN Ming-yue, et al. TGF-beta-Induced Upregulation of malat1 Promotes Bladder Cancer Metastasis by Associating with suz12 ［J］. Clinical Cancer Research, 2014, 20(6): 1531-1541. ［14］HIRATA H, HINODA Y, SHAHRYARI V, et al. Long noncoding RNA MALAT1 promotes aggressive renal cell carcinoma through Ezh2 and interacts with miR-205 ［J］. Cancer Research, 2015, 75(7): 1322-1331. ［15］Weber DG, Johnen G, Casjens S，et al. Evaluation of long noncoding RNA MALAT1 as a candidate blood-based biomarker for the diagnosis of non-small cell lung cancer ［Z］, 2013: 518. ［16］PANG Er-jun, YANG Rui, FU Xi-bo, et al. Overexpression of long non-coding RNA MALAT1 is correlated with clinical progression and unfavorable prognosis in pancreatic cancer ［J］. Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, 2015, 36(4): 2403-2407. ［17］YANG Min-hui, HU Zhi-yan, XU Chuan, et al. MALAT1 promotes colorectal cancer cell proliferation/migration/invasion via PRKA kinase anchor protein 9 ［J］. Biochimica et Biophysica acta, 2015, 1852(1): 166-174. ［18］MA Kang-xiao, WANG Hong-jie, LI Xiao-rong, et al. Long noncoding RNA MALAT1 associates with the malignant status and poor prognosis in glioma ［J］. Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, 2015, 36(5): 3355-3359. ［19］YANG L, BAI H-s, DENG Y, et al. High MALAT1 expression predicts a poor prognosis of cervical cancer and promotes cancer cell growth and invasion ［J］. European Review for Medical and Pharmacological Sciences, 2015, 19 (17): 3187-3193.

相似文献/References:

[1]胡玲,胡健蓉,徐凤仪,等.血管内皮生长因子在子宫内膜癌中的表达及其意义[J].中国计划生育和妇产科,2011,(01):0.
　HU Ling,HU Jian-rong,XU Feng-yi,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2011,(6):0.
[2]孙红敏,苑中甫.胰岛素样生长因子受体-1在子宫内膜癌组织中的表达及意义[J].中国计划生育和妇产科,2011,(02):0.
　SUN Hong-min,WAN Zhong-fu ..[J].Chinese Journal of Family Planning & Gynecotokology,2011,(6):0.
[3]田兆华,王建六.应客观评价腹膜后淋巴结切除在子宫内膜癌的治疗价值[J].中国计划生育和妇产科,2011,(03):0.
[4]陈晓丽.CD44v6、MMP-2在子宫内膜癌的表达及临床意义[J].中国计划生育和妇产科,2011,(03):0.
　CHEN Xiao-li.[J].Chinese Journal of Family Planning & Gynecotokology,2011,(6):0.
[5]梅彤,舒宽勇.CD133在子宫内膜癌中的研究进展[J].中国计划生育和妇产科,2012,(04):0.
　MEI Tong,SHU Kuan-yong* .Medical.[J].Chinese Journal of Family Planning & Gynecotokology,2012,(6):0.
[6]曹晓智,王小捷,李凤山,等.宫内膜细胞p16基因表达检测筛查子宫内膜癌的研究[J].中国计划生育和妇产科,2013,(06):0.
　Cao Xiao-zhi,Wang Xiao-jie,Li Feng-shan,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2013,(6):0.
[7]廖娟,杨菊生,李越,等.磁共振成像在Ⅰ、Ⅱ期子宫内膜癌术前分期中的应用分析[J].中国计划生育和妇产科,2014,(06):0.
　LIAO Juan,YANG Ju-sheng,LI Yue,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2014,(6):0.
[8]姜志欣,吴玉璘.女性人乳头瘤病毒感染及相关肿瘤[J].中国计划生育和妇产科,2014,(07):0.
[9]魏丽惠.关于子宫内膜癌筛查的思考[J].中国计划生育和妇产科,2015,(01):0.
[10]张超,蒋国庆.子宫内膜癌细胞学筛查的Meta分析[J].中国计划生育和妇产科,2015,(06):0.
　ZHANG Chao,JIANG Guo-qing,[J].Chinese Journal of Family Planning & Gynecotokology,2015,(6):0.
[11]韩翠香.长链非编码RNA MALAT1在子宫内膜癌中的表达及临床意义[J].中国计划生育和妇产科,2016,(06):0.
　HAN Cui-xiang,[J].Chinese Journal of Family Planning & Gynecotokology,2016,(6):0.

更新日期/Last Update: 1900-01-01