[1]贾晨阳,段华*,汪沙,等.子宫腺肌病EMI区VEGF与b-FGF的表达及其 与MVD的相关性分析[J].中国计划生育和妇产科,2017,(4):60- 64,67.
　JIA Chen-yang,DUAN Hua*,WANG Sha,et al.Expression of VEGF and b - FGF in EMI region of adenomyosis and its correlation with MVD[J].Chinese Journal of Family Planning & Gynecotokology,2017,(4):60- 64,67.
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子宫腺肌病EMI区VEGF与b-FGF的表达及其 与MVD的相关性分析
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2017年4期
页码:
60- 64,67
栏目:
论著与临床
出版日期:
2017-04-25

文章信息/Info

Title:
Expression of VEGF and b - FGF in EMI region of adenomyosis and its correlation with MVD
作者:
贾晨阳; 段华*; 汪沙; 李金皎
首都医科大学附属北京妇产医院妇科微创中心
Author(s):
JIA Chen-yang;  DUAN Hua*;  WANG Sha;  LI Jin-jiao
Gynecological Minimally Invasive Center, Capital Medical University, Beijing Maternity Hospital, Beijing 100006, P.R. China
关键词:
子宫腺肌病; 子宫内膜-肌层交界区; VEGF; b-FGF; 血管形成
Keywords:
adenomyosis;  endometrium-myometrial interface;  VEGF;  b-FGF;  angiogenesis
分类号:
R 453
摘要:
目的探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）和碱性成纤维细胞生长因子（basic-fibroblast growth factor,b-FGF） 在子宫腺肌病(adenomyosis，ADS)患者子宫内膜-肌层交界区(endometrial-myometrial interface,EMI)的表达及其与微血管密度（microvessel density，MVD）的相关性。方法选择2015年1月至2016年4月在首都医科大学附属北京妇产医院妇科微创中心因ADS行全子宫切除术的30例患者为ADS组(其中增殖期15例，分泌期15例)；同期因宫颈病变而行子宫切除的20例患者为对照组(其中增殖期10例，分泌期10例)。通过免疫组化方法分别检测两组EMI区及ADS组远肌层的VEGF、b-FGF及CD 34（标记微血管密度）蛋白的表达,分析3者间的相关性。结果① VEGF和b-FGF在ADS组及对照组EMI区的表达存在周期性变化。ADS组EMI区分泌期的表达（分别为3460±0322，3210±0364）高于增殖期（2940±0333，2880±0307），对照组EMI区分泌期的表达（分别为2080±0230，1980±0413）亦高于增殖期（1720±0326，1340±0362）（P均＜005）。而VEGF和b-FGF在ADS组远肌层的表达无明显周期性变化。② 无论增殖期还是分泌期,VEGF和b-FGF在ADS组EMI区的表达均高于远肌层和对照组EMI区（P均＜005）。③ MVD在两组EMI区及ADS组远肌层均呈周期性变化，其在ADS组EMI区分泌期计数（1121±290）高于增殖期（1022±285）条/40HP；在ADS组远肌层分泌期的计数（752±168）高于增殖期（615±125）条/40HP（P＜005）；其在对照组EMI区分泌期的计数（626±198）亦高于增殖期（575±101）条/40HP（P＜005）。无论增殖期还是分泌期,MVD计数在ADS组EMI区明显高于远肌层和对照组，差异均有统计学意义（P＜005）。④ VEGF和b-FGF在两组EMI区的表达均显著相关；且两者的表达与两组EMI区MVD计数亦分别呈正相关（P均<005）。结论VEGF和b-FGF在子宫腺肌病EMI区的表达明显增高，并且与MVD计数呈显著正相关。提示VEGF和b-FGF可能在ADS的血管形成及发生发展中起重要作用。
Abstract:
ObjectiveTo investigate the expression of vascular endothelial growth factor (VEGF) and basic-fibroblast growth factor (b-FGF) in the endometrial-myometrial interface (EMI) of patients with adenomyosis (ADS) and its relationship with microvessel density (MVD).MethodsSelect 30 ADS patients who had hysterectomy from January 2015 to April 2016 in Gynecological Minimally Invasive Center,Capital Medical University, Beijing Maternity Hospital as ADS group (15 cases of proliferative phase and 15 cases of secretory phase). 20 cases of hysterectomy due to cervical lesions were selected as control group (10 cases of proliferative phase and 10 cases of secretory phase).Immunohistochemical analysis of VEGF, b-FGF and CD34 (microvessel density) proteins was performed in the EMI and distal myometrium of the two groups. Results(1) The expression of VEGF and b-FGF in the EMI region of ADS group and control group was changed periodically. The expression of VEGF and b-FGF in the secretory phase of the EMI region of ADS group (3460 ± 0322, 232 ± 0364, respectively) was higher than that in the proliferative phase (2940 ± 0333,2880 ± 0307).The expression of VEGF and b-FGF in the EMI region of control group(2080±0230，1980±0413 ,respectively) was higher than that in the proliferative phase (1720 ± 0326,1340 ± 0362). The differences were statistically significant(P< 005). There was no significant change in the expression of VEGF and b-FGF in the distal myometrium of ADS group. （2）Whether in the proliferative or secretory period, the expression of VEGF and b-FGF in the EMI region of ADS group was higher than that in the distal muscle layer and the control group, the differences were statistically significant(P< 005). (3)MVD in the two groups’ EMI region and the distal muscle layer of ADS group showed a cyclical change. The secretory phase MVD in the EMI region of ADS group（752±168） was higher than that in the proliferative phase（1022±285）/40HP. The secretory phase MVD in distal muscle layer of ADS group（752±168）was higher than that in the proliferative phase（615±125）/40HP. The differences were statistically significant(P< 005). The MVD counts were significantly higher in the EMI region of the ADS group than in the distal muscle and the control group (P<005), both in the proliferative phase and the secretory phase.(4) VEGF and b-FGF were significantly correlated in the two groups in EMI regions. Their expression was positively correlated with the MVD counts in the EMI region of the two groups（P＜005）.ConclusionThe expression of VEGF and b-FGF in EMI region of ADS was significantly increased, and positively correlated with MVD. These results suggest that VEGF and b-FGF may play an important role in the development of ADS

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备注/Memo

备注/Memo:
国家自然科学基金（项目编号：81270680，81571412）；北京市医院管理局重点医学发展专项（项目编号：ZYLX201406）；北京市自然科学基金资助项目（项目编号：7142056）
更新日期/Last Update: 2017-04-25