[1]王玉清.基质金属蛋白酶-2、凋亡抑制基因、白介素-6和信号传导及转录活化因子3在子痫前期患者胎盘中的检测水平及意义[J].中国计划生育和妇产科,2018,(10):62-67.
　WANG Yu-qing.Detection and significance of MMP-2, Survivin, IL-6, and STAT3 in placenta of patients with preeclampsia[J].Chinese Journal of Family Planning & Gynecotokology,2018,(10):62-67.
点击复制

基质金属蛋白酶-2、凋亡抑制基因、白介素-6和信号传导及转录活化因子3在子痫前期患者胎盘中的检测水平及意义
分享到：

《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年10期
页码:
62-67
栏目:
论著与临床
出版日期:
2018-10-25

文章信息/Info

Title:
Detection and significance of MMP-2, Survivin, IL-6, and STAT3 in placenta of patients with preeclampsia
作者:
王玉清
乐陵市人民医院妇产科
Author(s):
WANG Yu-qing
Department of Gynaecology and Obstetrics,Leling People's Hospital, Dezhou Shandong 253600,P.R.China
关键词:
子痫前期; 胎盘组织; 基质金属蛋白酶-2; 凋亡抑制基因; 白介素-6; 信号传导及转录活化因子3
Keywords:
preeclampsia; pacenta tissue; MMP-2; Survivin;  IL-6; STAT 3
分类号:
R 714.24+5
摘要:
目的探究基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）、凋亡抑制基因（Survivin）、白介素-6（interleukin-6，IL-6）、信号传导及转录活化因子3（signal transducers and activators of transcription 3，STAT 3）在子痫前期患者胎盘中的水平及意义。方法选取乐陵市人民医院2016年3月至2017年5月收治的76例子痫前期孕妇为研究对象，按照病情分为轻度子痫前期组（34例）与重度子痫前期组（42例），另选取同期35例正常孕妇为对照组，分娩后收集胎盘组织，HE染色观察各组胎盘组织变化，免疫组化法检测胎盘组织中MMP-2、Survivin、IL-6和STAT 3表达，Pearson相关性分析MMP-2、Survivin、IL-6、和STAT 3与临床指标的相关性。结果子痫前期组收缩压、舒张压、尿蛋白均高于对照组，新生儿体重低于对照组，且随着病情程度加重，重度子痫前期组变化程度更为显著（P＜005）；免疫组化显示，MMP-2、Survivin、STAT 3在3组中阳性表达量、平均光密度逐渐降低，IL-6阳性表达量、平均光密度逐渐增加(P＜005)；收缩压、舒张压、尿蛋白与MMP-2、Survivin、STAT 3呈负相关，与IL-6呈正相关；新生儿体重与MMP-2、Survivin、STAT 3呈正相关，与IL-6呈负相关（P＜005）。结论MMP-2、Survivin、STAT 3低表达，IL-6高表达与子痫前期发生发展、新生儿的预后有关。
Abstract:
ObjectiveTo explore the levels and significance of matrix metalloproteinase-2(MMP-2),apoptosis inhibitor gene(Survivin),interleukin6(IL6),signal transducer and activator of transcription 3(STAT 3) in placenta of patients with preeclampsia. Methods76 cases of pre-eclampsia pregnant women from March 2016 to May 2017 in Leling People's Hospital were selected as the study subjects.According to the condition, 34 cases were in the mild preeclampsia group,42 cases in the severe preeclampsia group,and 35 normal pregnant woman were the control group. Placental tissues were collected after delivery, and the changes of placental tissue were observed by HE staining. The expressions of MMP2, Survivin, IL6 and STAT 3 in placenta were detected by immunohistochemistry. Pearson correlation analysis was performed on MMP2, Survivin and IL6,STAT 3 and the correlation with clinical indicators.ResultsThe systolic blood pressure, diastolic blood pressure and urine protein in the preeclampsia group were higher than those in the control group. The neonatal weight was lower than that in the control group, and the severity of the severe preeclampsia group was more significant(P<005). Immunohistochemistry showed that the positive expression and average optical density of MMP2, Survivin and STAT 3 decreased gradually, and the positive expression of IL6 and the average optical density increased gradually (P<005).Systolic blood pressure, diastolic blood pressure, urine protein were significantly negatively correlated with MMP2, Survivin and STAT 3, and positively correlated with IL6. Neonatal weight was significantly positively correlated with MMP2, Survivin and STAT 3, and IL6 showed a significant negative correlation (P<005).ConclusionLow expression of MMP-2, Survivin and STAT 3, high expression of IL-6 is associated with the development of preeclampsia and the prognosis of neonates.

参考文献/References:

［1］段涛．子痫前期-子痫相关问题近十年的回顾与展望 ［J］．中华妇产科杂志，2013，48（4）：268-271． ［2］罗欣，漆洪波．子痫前期发病机制分子生物学研究进展 ［J］．中国实用妇科与产科杂志，2012，28（4）：309-311． ［3］刘芳．IL-6及Survivin在胚胎停育中的临床意义 ［J］．中国妇幼保健，2015，30（22）：3882-3884． ［4］乐杰．妇产科学 ［M］第7版．北京：人民卫生出版社，2008． ［5］曾雅畅，陈悦．早发型子痫前期预后因素的Logistic回归分析 ［J］．中国妇幼保健，2013，28（36）：5957-5960． ［6］CANAKCI V, CANAKCI C F, CANAKCI H, et al. Periodontal disease as a risk factor for pre-eclampsia: a case control study ［J］. The Australian & New Zealand Journal of Obstetrics & Gynaecology, 2004, 44 (6): 568-573. ［7］SULISTYOWATI S. The role of oxidative stress in the pathogenesis of pre-eclampsia ［J］. Pregnancy Hypertension, 2014, 4 (3): 244. ［8］李爱金，章伟丽，陈再玲，等．子痫前期胎盘组织氧化应激和炎症反应状态及与新生儿预后的关系 ［J］．实用医学杂志，2013，29（20）：3365-3367． ［9］周小波，石书明，张华．线粒体自噬受损引起的ROS清除障碍导致子痫前期患者滋养细胞过度凋亡 ［J］．重庆医科大学学报，2017，42（3）：315-318． ［10］秦兴发，张华，漆洪波，等．缺氧诱导滋养细胞自噬参与子痫前期发病的实验研究 ［J］．重庆医学，2014，43（5）：569-571． ［11］齐朝阳，高宇琳，黎琳欢，等．促红细胞生成素及其受体、微血管密度在重度子痫前期胎盘中的作用 ［J］．实用医学杂志，2014，30（3）：425-428． ［12］TYURENKOV I N, PERFILOVA V N, SMIRNOV A V, et al. Features of endothelial dysfunction and morphofunctional changes of the uteroplacental complex in experimentally induced pre-eclampsia ［J］. Pregnancy Hypertension: an International Journal of Women's Cardiovascular Health, 2016, 6 (4): 423-430. ［13］DESMEULES P, TRUDEL D, TURCOTTE S, et al. Prognostic significance of TIMP-2, MMP-2, and MMP-9 on high-grade serous ovarian carcinoma using digital image analysis ［J］. Human Pathology, 2015, 46 (5): 739-745. ［14］RICCI S, BRUZZESE D, DI CARLO A. Evaluation of MMP-2, MMP-9, TIMP-1, TIMP-2, NGAL and MMP-9/NGAL complex in urine and sera from patients with bladder cancer ［J］. Oncology Letters, 2015, 10 (4): 2527-2532. ［15］GONZALEZ-PUEBLA E, GONZALEZ-HORTA C, INFANTE-RAMIREZ R， et al. Altered expressions of MMP-2, MMP-9, and TIMP-2 in placentas from women exposed to Lead ［J］. Human & Experimental Toxicology, 2012, 31 (7): 662-670. ［16］底建敏，崔文华，王键，等．Xiap和Survivin在子痫前期患者胎盘组织中的表达及意义 ［J］．中国妇幼保健，2014，29（8）：1275-1277． ［17］钟薇．子痫前期胎盘组织中visfatin、Xiap和Survivin表达量检测及其与血清学指标的关系 ［J］．海南医学院学报，2015，21（9）：1251-1253，1256． ［18］WHITEHEAD C L, WALKER S P, LAPPAS M, et al. Circulating RNA coding genes regulating apoptosis in maternal blood in severe early onset fetal growth restriction and pre-eclampsia ［J］. Journal of Perinatology : Official Journal of the California Perinatal Association, 2013, 33 (8): 600-604. ［19］司斯，孟涛．子痫前期孕妇白细胞介素6、17和24水平的变化及临床意义 ［J］．中国医科大学学报，2016，45（6）：503-505． ［20］张展，刘丽莎，高峻峻，等．滋养细胞中STAT 3活性与MMP-9表达水平的相关性 ［J］．中国妇幼保健，2013，28（34）：5709-5711． ［21］杜丽荣，张素芝，马战欣，等．STAT 3在正常早孕妇女与反复自然流产患者绒毛和蜕膜中的表达 ［J］．中国妇幼保健，2010，25（23）：3303-3304． ［22］林赠华，刘红，姜胜华，等．非重型再生障碍性贫血患者血清对32 D细胞凋亡及Stat 3蛋白磷酸化的影响 ［J］．山东医药，2011，51（37）：16-18．

相似文献/References:

[1]宋改让,傅晓冬,刘华梅,等.IL-6、CRP在子痫前期中的意义[J].中国计划生育和妇产科,2009,(05):0.
　SONG Gai-rang,FU Xiao-dong,LIU Hua-meiThe department of gynaecology and obstetrics,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2009,(10):0.
[2]李延杰.早发型重度子痫前期妊娠116例结局分析[J].中国计划生育和妇产科,2010,(02):0.
　LI Yan-jie.[J].Chinese Journal of Family Planning & Gynecotokology,2010,(10):0.
[3]赵昆.D-二聚体测定在产科常见病理性高凝状态疾病的临床意义[J].中国计划生育和妇产科,2011,(04):0.
　ZHAO Kun.[J].Chinese Journal of Family Planning & Gynecotokology,2011,(10):0.
[4]张峰,丁金云.子痫前期患者血清瘦素、sFlt-1/PLGF、C-反应蛋白及IP-10关系的研究[J].中国计划生育和妇产科,2012,(06):0.
　ZHANG Feng,DING Jin-yun.[J].Chinese Journal of Family Planning & Gynecotokology,2012,(10):0.
[5]乔宠,杨小梅,林其德,等.子痫前期的流行病学研究进展[J].中国计划生育和妇产科,2013,(06):0.
　QIAO Chong,YANG Xiao-mei,LIN Qi-de,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[6]龚云辉,周容.子痫前期的预测和预警[J].中国计划生育和妇产科,2013,(06):0.
　GONG Yun-hui,ZHOU Rong,[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[7]顾宁,胡娅莉.子痫前期的预测和预防引发的思考[J].中国计划生育和妇产科,2013,(06):0.
　GU Ning,HU Ya-li,[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[8]郭宏霞,杨孜.子痫前期发病时期对母儿影响[J].中国计划生育和妇产科,2013,(06):0.
　GUO Hong-xia,YANG Zi,[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[9]宋亦军,刘俊涛.炎症与子痫前期[J].中国计划生育和妇产科,2013,(06):0.
　SONG Yi-jun,LIU Jun-tao,[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[10]乐怡平,林建华.子痫前期转运与分娩处理[J].中国计划生育和妇产科,2013,(06):0.
　LE Yi-ping,LIN Jian-hua,[J].Chinese Journal of Family Planning & Gynecotokology,2013,(10):0.
[11]方颖.干扰素诱导蛋白16在子痫前期孕妇胎盘组织中 表达水平及其与滋养细胞侵袭和胎盘缺氧的相关性分析[J].中国计划生育和妇产科,2018,(11):63.
　FANG Ying.Expression of IFI 16 in placental tissue of preeclampsia and its correlation with trophoblast invasion and placental anoxia[J].Chinese Journal of Family Planning & Gynecotokology,2018,(10):63.

更新日期/Last Update: 2018-10-25