[1]王菲菲,马小星*.乙肝表面抗原和乙肝e抗原均阳性产妇乙肝病毒宫内感染情况及与Toll样受体4和干扰素-γ的相关性分析[J].中国计划生育和妇产科,2018,(10):78-80.
　WANG Fei-fei,MA Xiao-xing*.HBV intrauterine infection in HBsAg and HBeAg positive women and its correlation with TLR4 and interferon-γ[J].Chinese Journal of Family Planning & Gynecotokology,2018,(10):78-80.
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乙肝表面抗原和乙肝e抗原均阳性产妇乙肝病毒宫内感染情况及与Toll样受体4和干扰素-γ的相关性分析
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年10期
页码:
78-80
栏目:
论著与临床
出版日期:
2018-10-25

文章信息/Info

Title:
HBV intrauterine infection in HBsAg and HBeAg positive women and its correlation with TLR4 and interferon-γ
作者:
王菲菲; 马小星*
延安市人民医院中心产房
Author(s):
WANG Fei-fei; MA Xiao-xing*
Central Delivery Room,Yan'an People's Hospital, Yan'an Shaanxi 716000,P.R.China
关键词:
乙型肝炎病毒; 宫内感染; 干扰素; Toll样受体4; 相关性
Keywords:
hepatitis B virus; intrauterine infection; interferon ; Toll Like Receptor 4; relativity
分类号:
R 714.7
摘要:
目的分析探讨乙肝表面抗原（hepatitis B surface antigens，HBsAg）和乙型肝炎e抗原（hepatitis B e antigens，HBeAg）均阳性产妇乙型肝炎病毒（hepatitis B virus,HBV）宫内感染情况及与Toll样受体4（Toll-like receptor 4, TLR 4）和干扰素-γ的相关性。方法回顾性分析延安市人民医院2015年1月至2017年2月收治的198例HBsAg和HBeAg均阳性产妇的临床资料，根据产妇是否出现HBV宫内感染将其分为甲乙两组，有HBV宫内感染的100例为甲组，无HBV宫内感染的98例为乙组。分别测定两组产妇分娩前的外周血HBV DNA含量和外周血干扰素-γ水平，分娩后脐带血中HBeAg和HBsAg水平，利用免疫组化测定两组产妇胎盘TLR 4和干扰素-γ等的表达情况。结果分娩前甲组产妇干扰素-γ水平［（21238±3549）pg/mL］低于乙组产妇［（1 27939±12638）pg/mL］（P＜005）；甲组产妇的HBV DNA水平［（636±036）×103copies/mL］高于乙组产妇［（018±003）×103copies/mL］（P＜005）。甲组产妇分娩后脐带血中的HBeAg和HBsAg水平分别为（223±031）PEI U/mL和（341±032）ng/mL，均高于乙组产妇的（032±012）PEI U/mL和（030±009）ng/mL（P＜005）。甲组产妇分娩后的脐带血中TLR 4和相应mRNA以及干扰素-γ水平分别为（3649±612）pg/mL、（071±013）pg/mL、（7917±1098）pg/mL，均低于乙组产妇的（4932±718）pg/mL、（097±012）pg/mL、（11438±1892）pg/mL（P＜005）。结论干扰素-γ和TLR4在抑制HBsAg和HBeAg均阳性产妇HBV宫内感染中起重要作用，对于预防和控制HBV孕产妇宫内感染具有较高的临床价值。
Abstract:
ObjectiveTo analyze hepatitis B surface antigens(HBsAg) and hepatitis B e antigens(HBeAg) positive for maternal hepatitis B virus(HBV) intrauterine infection and their correlation of TLR4 and interferon-γ(INF-γ). MethodsRetrospectively analyzed the clinical data of 198 HBsAg and HBeAg-positive women admitted to Yan'an People's Hospital from January 2015 to February 2017.According to whether the mother had HBV intrauterine infection,they were divided into two groups. 100 cases(group A) were with HBV intrauterine infection, and 98 cases(group B)were without HBV intrauterine infection. The peripheral blood HBV-DNA content and peripheral blood interferon-γ levels were measured before delivery. The levels of HBeAg and HBsAg in the cord blood of the two groups were measured. The immunological histochemistry was used to determine the maternal placental Toll-like expression of receptor 4 and interferon-γ, etc.ResultsThe interferon-γ level of the group A was (21238±3549) pg/mL before delivery, which was significantly lower than that of the group B (1 27939±12638) pg/mL (t=78282, P<005). The level of HBV DNA in group A was (636±036)×103 copies/mL, which was significantly higher than that in group B (018±003)×103 copies/mL (t=236383, P<005). The levels of HBeAg and HBsAg in umbilical cord blood of group A were (223±031) PEI U/mL and (341±032) ng/mL, respectively, which were significantly higher than those of group B (032±012) PEI U/ mL and (030 ± 009) ng/mL (t=64265, 93382, P<005).The levels of TLR4, mRNA and INF-γ in umbilical cord blood of group A were(3649±612) pg/mL,(071±013)pg/mL,(7917±1098)mg/mL, respectively, which were significantly lower than those of group B (4932±718)pg/mL,(097±012)pg/mL,(11438±1892)pg/mL(P＜005).ConclusionInterferon-γ and Toll-like receptor 4 play an important role in inhibiting HBV intrauterine infection in both HBsAg and HBeAg-positive women, and have high clinical value for preventing and controlling HBV maternal intrauterine infection.

参考文献/References:

［1］王素华，万建玉．Toll样受体4及干扰素-γ与乙肝病毒宫内感染相关性分析 ［J］．中国医刊，2016，51（5）：82-84． ［2］GYAWALI R, BHAYAL B K, ADHIKARY R, et al Retrospective data on causes of childhood vision impairment in Eritrea ［J］. BMC Ophthalmology, 2017, 17 (1): 209. ［3］ZHANG Li-xia, SUN Yang, ZHAO Hai, et al. A bayesian stepwise discriminant model for predicting risk factors of preterm premature rupture of membranes: a case-control study ［J］. Chinese Medical Journal, 2017, 130 (20): 2416-2422. ［4］SANTA RITA T H, BARRA R B, PEIXOTO G P, et al. Association between suspected Zika virus disease during pregnancy and giving birth to a newborn with congenital microcephaly: a matched case-control study ［J］. BMC Research Notes, 2017, 10 (1): 457. ［5］LEVINE D. Invited commentary on "central nervous system effects of intrauterine Zika virus infection" ［J］. Radiographics : a Review Publication of the Radiological Society of North America, Inc, 2017, 37 (6): 1850-1853. ［6］SHARP G C, SALAS L A, MONNEREAU C, et al. Maternal BMI at the start of pregnancy and offspring epigenome-wide DNA methylation: findings from the pregnancy and childhood epigenetics (PACE) consortium ［J］. Human Molecular Genetics, 2017, 26 (20): 4067-4085. ［7］高怡，郭健，付振东，等．HBV宫内感染新生儿外周血调节性T细胞表达 ［J］．中国公共卫生，2013，29（4）：488-490． ［8］HEIDER A. Fetal vascular malperfusion ［J］. Archives of Pathology & Laboratory Medicine, 2017, 141 (11): 1484-1489. ［9］SANO M, NEMOTO K, MIURA T, et al. Endoscopic treatment of intrauterine device migration into the bladder with stone formation ［J］. Journal of Endourology Case Reports, 2017, 3 (1): 105-107. ［10］龙禹，黄星，陈悦，等．HBVDNA阳性孕妇TLR4及IL-12、IL-4与HBV宫内感染关系的研究 ［J］．中国妇幼保健，2014，29（8）：1174-1177． ［11］ AAS-ENG M K, LANGEBREKKE A, HUDELIST G. Complications in operative hysteroscopy is prevention possible? ［J］. Acta Obstetricia et Gynecologica Scandinavica, 2017, 96 (12): 1399-1403. ［12］KUMAR N, DAS V, AGARWAL A, et al. Fetomaternal outcomes in pregnant women with hepatitis E infection： still an important fetomaternal killer with an unresolved mystery of increased virulence in pregnancy ［J］. Turkish Journal of Obstetrics and Gynecology, 2017, 14 (2): 106-113. ［13］唐洁，许联红，陈洁，等．新生儿免疫预防乙型肝炎病毒母婴传播的效果及传播易患因素分析 ［J］．中国妇幼保健，2014，29（21）：3427-3430． ［14］Saakyan SV, Katargina LA, Krichevskaya GI, et al. Specific immunoglobulins G and M in blood serum in retinoblastoma and 'pseudoretinoblastoma' ［J］. Vestn Oftalmol, 2017,133 (4):12-16. ［15］彭建平，孙克伟，银思涵，等．HBV对健康产妇脐带血浆细胞样树突状细胞Toll样受体9、干扰素调节因子7、干扰素α表达的影响 ［J］．临床肝胆病杂志，2016，32（5）：894-898． ［16］AGRA I K, AMORIM FILHO A G, LIN L H, et al. Parameters associated with adverse fetal outcomes in parvovirus B19 congenital infection ［J］. Revista Brasileira de Ginecologia e Obstetricia : Revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia, 2017, 39 (11): 596-601. ［17］张传涛，郭尹玲，黄群，等．HH方治疗HBV转基因小鼠抗病毒作用及其对TLR 3/TLR 4的影响 ［J］．实用医学杂志，2016，32（23）：3841- 3844． ［18］CASILLAS-VEGA N, MORFN-OTERO R, GARCA S, et al. Frequency and genotypes of Chlamydia trachomatis in patients attending the obstetrics and gynecology clinics in Jalisco, Mexico and correlation with sociodemographic, behavioral, and biological factors ［J］. BMC Women's Health, 2017, 17 (1): 83.

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更新日期/Last Update: 2018-10-25