[1]刘耀丹,洪莉*,李洋,等.电刺激对小鼠成纤维细胞L 929机械力损伤的 保护作用[J].中国计划生育和妇产科,2018,(12):23-26.
　LIU Yao-dan,HONG Li*,LI Yang,et al.Protective effects of electrical stimulation on mechanical stress-induced oxidative damage in mouse fibroblast L 929 cells[J].Chinese Journal of Family Planning & Gynecotokology,2018,(12):23-26.
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电刺激对小鼠成纤维细胞L 929机械力损伤的 保护作用
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年12期
页码:
23-26
栏目:
论著与临床
出版日期:
2018-12-25

文章信息/Info

Title:
Protective effects of electrical stimulation on mechanical stress-induced oxidative damage in mouse fibroblast L 929 cells
作者:
刘耀丹; 洪莉*; 李洋; 李素廷
武汉大学人民医院妇产科
Author(s):
LIU Yao-dan;  HONG Li*;  LI Yang;  LI Su-ting
Department of Gynecology and Obstetrics, People’s Hospital of Wuhan University, Wuhan Hubei 430060, P.R.China
关键词:
成纤维细胞L 929; 电刺激; 机械力损伤; 细胞活性氧; Caspase-3
Keywords:
mouse fibroblasts L 929 cells;  electrical stimulation;  mechanical stress;  reactive oxygen species; Caspase-3
分类号:
R 711.2
摘要:
目的探讨电刺激（electrical stimulation，ES）对机械力诱导的小鼠成纤维细胞L 929氧化损伤的保护作用。方法将小鼠成纤维细胞L 929分为正常对照组、电刺激组、加力模型组和加力后电刺激组。采用CCK-8法检测细胞活性，间接化学荧光法检测细胞活性氧（reactive oxygen species, ROS）含量变化，JC-1染色法检测细胞内线粒体膜电位（mitochondrial membrane potential, MMP）变化，分光光度法测定Caspase-3酶活性变化。结果加力组细胞活性明显下降，细胞内ROS增加，线粒体膜电位下降，Caspase-3酶活性增强，差异均有统计学意义（P＜005）。与加力组相比，加力后电刺激组细胞活性增强，线粒体膜电位水平上升，细胞内ROS含量减少，Caspase-3酶活性减弱，差异均有统计学意义（P＜005）。结论电刺激可通过维持细胞活性、减少细胞凋亡、减少细胞内ROS含量及Caspase-3酶活性，而对机械力诱导的细胞氧化损伤起到一定的保护作用。
Abstract:
ObjectiveTo explore the protective effects of electrical stimulation on mechanical stress-induced oxidative damage in mouse fibroblast L 929 cells. MethodsThe mouse fibroblast L 929 cells were divided into four groups: blank control group, electrical stimulation group, mechanical stress group and electrical stimulation after mechanical stress group. The cell viability and cellular senescent state were analyzed using CCK-8. Fluorometric assays were performed to detect the formation of reactive oxygen species (ROS) and mitochondrial membrane potential detection (JC-1) staining in the cells. The caspase-3 activity was detected by Caspase-3 Activity Assay Kit. ResultsThe cell viability in mechanical stress group decreased significantly, intracellular ROS increased, mitochondrial membrane potential decreased, and Caspase-3 enzyme activity increased. The differences were statistically significant (P<005). Compared with mechanical stress group, the cell viability in electrical stimulation after mechanical stress group increased, the mitochondrial membrane potential increased, the intracellular ROS content decreased, and the Caspase-3 enzyme activity weakened. The differences were statistically significant (P<005). ConclusionElectrical stimulation can maintain cell viability, reduce cell apoptosis, reduce intracellular ROS and Caspase-3 activity. Therefore,the electrical stimulation may be a potential method for the prevention and treatment of mechanical stress-induced cell injury.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然基金（面上项目）（项目编号：81771562）
更新日期/Last Update: 2018-12-25