[1]张杨,洪莉*.宫腔粘连患者子宫内膜组织跨膜前列腺雄激素诱导蛋白1和转化生长因子β1的表达情况[J].中国计划生育和妇产科,2018,(8):22-25.
　ZHANG Yang,HONG Li*.The expression of PMEPA1 and TGF-β1 in endometrium of patients with intrauterine adhesions[J].Chinese Journal of Family Planning & Gynecotokology,2018,(8):22-25.
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宫腔粘连患者子宫内膜组织跨膜前列腺雄激素诱导蛋白1和转化生长因子β1的表达情况
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2018年8期
页码:
22-25
栏目:
论著与临床
出版日期:
2018-08-25

文章信息/Info

Title:
The expression of PMEPA1 and TGF-β1 in endometrium of patients with intrauterine adhesions
作者:
张杨; 洪莉*
武汉大学人民医院妇产科
Author(s):
ZHANG Yang; HONG Li*
Department of Gynecology and Obstetrics,Renmin Hospital of Wuhan University,Wuhan Hubei 430060,P.R.China
关键词:
PMEPA1; TGF-β1; 宫腔粘连; 病理机制
Keywords:
PMEPA1; TGF-β1; intrauterine adhesions; pathomechanism
分类号:
R 711.32
摘要:
目的探讨跨膜前列腺雄激素诱导蛋白1 (prostate transmembrane protein androgen induced 1，PMEPA 1)和转化生长因子β1（transforming growth factor-β1,TGF-β1）在宫腔粘连（intrauterine adhesions，IUA）患者子宫内膜组织中的表达量及相关性。方法选取武汉大学人民医院妇产科2016年6月至2017年12月因IUA行宫腔镜手术治疗的患者50例为粘连组，20例因不孕症行宫腔镜手术的非IUA患者为对照组，采集两组患者子宫内膜组织，根据粘连的评分标准评分并分级，粘连组又分为轻度组（10例）、中度组（19例）、重度组（21例），收集的内膜组织采用qRT-PCR和Western blot检测PMEPA 1和TGF-β1的mRNA和蛋白的表达量并在蛋白表达水平作两者相关性分析。结果①　PMEPA 1 mRNA的表达：中、重度粘连组低于对照组和轻度粘连组，重度组低于中度组，差异均有统计学意义（t=7719、1284、2614, P＜00001、＜00001、＜005）；轻度组与对照组比较差异无统计学意义(P＞005)。②　PMEPA 1蛋白的表达：轻、中、重度粘连组均低于对照组（t=2886、6669、1547，P＜005、＜0001、＜00001)，重度组低于中度组（t=5119, P＜00001）。③　TGF-β1的mRNA和蛋白的表达：粘连组高于对照组（t=4988、8816、90514, P均＜0001）。④　PMEPA 1和TGF-β1在中、重度组中呈负相关关系（r=-06342、-08407,P=00035、＜00001）。结论PMEPA 1和TGF-β1可能参与IUA的发生发展.
Abstract:
ObjectiveTo detect the expression of PMEPA1 and TGF-β1 in endometrium of patients with intrauterine adhesions(IUA) and analyze the correlation between the two indexes. Methods50 IUA patients and 20 non-IUA patients(control group) were selected from Renmin Hospital of Wuhan University from June 2016 to December 2017 to acquire the endometrium. According to the points-scoring system of AFS, the IUA patients were divided into 3 groups, the light group(10 patients), the moderate group(19 patients) and the severe group(21 patients).Rt-RCR and Western blot were used to detecte the mRNA and protein of PMEPA1 and TGF-β1 and analyze the correlation between the two indexes at protein level. Results① The expression of PMEPA 1 mRNA was lower in the moderate and severe adhesion group than in the control group and the mild adhesion group, and the severe group was lower than the moderate group (t=7719, 1284, 2614,P<00001，P＜00001,＜005). ② PMEPA 1 protein expression: the light, moderate, and severe adhesion groups were lower than the control group(t=2886, 6669, 1547,P<005,<0001,<00001), and the severe group was lower than the middle group(t=5119,P<00001). ③ mRNA and protein expression of TGF-β1: The adhesion group was higher than the control group(t=4988, 8816, 90514,P all<0001).④ PMEPA1 and TGF-β1 were negatively correlated in the moderate and severe groups(r=-06342, -08407, P=00031,<00001). ConclusionPMEPA1 and TGF-β1 may participate in the occurrence and development of IUA.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（项目编号：81771562）
更新日期/Last Update: 2018-08-25