[1]王莹莹,王君敏,陈璐璐,等.Wnt/β-catenin信号通路及WNK 1基因在盆腔脏器脱垂发病机制中的作用[J].中国计划生育和妇产科,2019,(5):56-61.
　WANG Ying-ying,WANG Jun-min,CHEN Lu-lu,et al.The role of Wnt/β-catenin signaling pathways and WNK 1 in the pathogenesis of pelvic organ prolapse[J].Chinese Journal of Family Planning & Gynecotokology,2019,(5):56-61.
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Wnt/β-catenin信号通路及WNK 1基因在盆腔脏器脱垂发病机制中的作用
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2019年5期
页码:
56-61
栏目:
论著与临床
出版日期:
2019-05-25

文章信息/Info

Title:
The role of Wnt/β-catenin signaling pathways and WNK 1 in the pathogenesis of pelvic organ prolapse
作者:
王莹莹; 王君敏; 陈璐璐; 徐锋; 王肖帆; 乔艳华; 张志磊; 赵冰*
郑州大学第三附属医院妇产科
Author(s):
WANG Ying-ying; WANG Jun-min; CHEN Lu-lu; XU Feng; WANG Xiao-fan; QIAO Yan-hua; ZHANG Zhi-lei; ZHAO Bing*
Department of Obstetrics and Gynaecology,The Third Affiliated Hospital of Zhengzhou University,Zhengzhou Henan 450052,P.R.China
关键词:
盆腔脏器脱垂; WNK 1基因; Wnt经典信号通路; 分子机制
Keywords:
pelvic organ prolapse; Wnt/β-catenin signaling pathway; WNK 1 gene; molecular mechanism
分类号:
R 711.23
摘要:
目的研究盆腔脏器脱垂(pelvic organ prolapse,POP)患者中Wnt经典信号通路（Wnt/β-catenin通路）、WNK 1基因的表达变化及两者相关性，初步探讨POP发生发展的可能分子机制。方法选择2017年1月至2018年5月在郑州大学第三附属医院因POP行阴式子宫切除术患者27例（POP组），及同期因妇科良性疾病行腹式或阴式子宫切除术患者21例（对照组）为研究对象。术中取子宫两侧主韧带组织，HE染色观察平滑肌束及成纤维细胞，Masson染色观察胶原纤维；WB及PCR检测通路相关蛋白（Wnt16、β-catenin、FZD 5）、WNK 1及Ⅰ型胶原蛋白(COL 1)的表达，并分析其与POP病理特征的关系，同时分析WNK 1基因与Wnt信号通路的相关性。结果① HE染色显示POP组平滑肌束萎缩断裂，成纤维细胞排列紊乱；② Masson染色可见胶原纤维明显受损、含量减少；③ Western-blot及Real-TimePCR显示POP组Wnt 16、β-catenin、WNK 1、COL 1等mRNA及蛋白水平均明显下降，FZD 5 mRNA水平表达上升，蛋白水平表达无差异；WNK 1基因与Wnt通路表达趋势呈正相关；随着绝经时间延长及脱垂级别增加，β-catenin、WNK 1及COL 1表达下降。结论POP的发生发展可能与WNK 1激酶水平及Wnt经典信号通路表达下降有关，β-catenin、WNK 1及COL 1的表达与绝经时间、脱垂程度有关。
Abstract:
ObjectiveTo investigate the expression of Wnt classical signaling pathway (Wnt/β-catenin pathway) and WNK 1 gene in patients with pelvic organ prolapse (POP) and the correlation between them, and to explore the possible molecular mechanism of POP development. Methods27 patients (POP group) who underwent POP vaginal hysterectomy at The Third Affiliated Hospital of Zhengzhou University from January 2017 to May 2018, and 21 patients who underwent abdominal or vaginal hysterectomy for benign gynecological diseases in the same period (control group) were the subject of the study.The main ligament tissues on both sides of the uterus were taken during operation. Smooth muscle bundles and fibroblasts were observed by HE staining, and collagen fibers were observed by Masson staining. WB and PCR detect pathway-related proteins (Wnt16, β-catenin, FZD 5), WNK 1 and type I collagen (COL 1) expression, and analyze its relationship with pathological features of POP, and analyze the correlation between WNK 1 gene and Wnt signaling pathway .Results ① HE staining showed that the smooth muscle bundle atrophy and rupture in the POP group, and the fibroblasts were disorderly arranged;② Masson staining showed that collagen fibers were significantly damaged and the content was reduced;③ Western-blot and Real-Time PCR showed that the mRNA and protein levels of Wnt 16, β-catenin, WNK 1, COL 1, etc. in POP group decreased significantly, and the expression of FZD 5 mRNA level increased, and there was no difference in protein expression. The expression of WNK 1 gene was positively correlated with the expression of Wnt pathway. The expression of β-catenin, WNK 1 and COL 1 decreased with the prolongation of menopause and the increase of prolapsed. ConclusionThe development of POP may be related to the decrease of WNK 1 kinase level and the expression of Wnt classical signaling pathway. The expression of β-catenin, WNK 1 and COL 1 is related to menopausal time and degree of prolapse.

参考文献/References:

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备注/Memo

备注/Memo:
国家青年基金项目（项目编号：81300469 ）；河南省高校创新人才计划（项目编号：18HASTIT045）
更新日期/Last Update: 2019-05-25