[1]洪莎莎,李洋,李秉枢,等.microRNA21对机械力作用下小鼠L929细胞增殖和凋亡的影响[J].中国计划生育和妇产科,2020,(4):64-67.
　HONG Sha sha,LI Yang,LI Bing shu,et al.Effect of microRNA21 on the proliferation and apoptosis of mouse L929 fibroblast after mechanical strain[J].Chinese Journal of Family Planning & Gynecotokology,2020,(4):64-67.
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microRNA21对机械力作用下小鼠L929细胞增殖和凋亡的影响
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2020年4期
页码:
64-67
栏目:
论著与临床
出版日期:
2020-04-25

文章信息/Info

Title:
Effect of microRNA21 on the proliferation and apoptosis of mouse L929 fibroblast after mechanical strain
作者:
洪莎莎; 李洋; 李秉枢; 刘成; 闵洁; 胡鸣; 汤剑明; 洪莉*
武汉大学人民医院妇二科
Author(s):
HONG Shasha;  LI Yang; LI Bingshu; LIU Cheng; MIN Jie; HU Ming; TANG Jianming;  HONG Li*
2nd Department of Gynecology，Renmin Hospital of Wuhan University,Wuhan Hubei 430060,P.R.China
关键词:
压力性尿失禁; 小鼠成纤维细胞; miRNA21; 细胞增殖; 细胞凋亡
Keywords:
stress urinary incontinence; L929 cell line; miRNA21; cell proliferation;  cell apoptosis
分类号:
R 694+54
摘要:
目的 探讨miRNA21对机械力作用下小鼠成纤维细胞系L929细胞的增殖和凋亡的影响。方法 细胞培养与转染将L929细胞接种于细胞培养基（RPMI1640）中，实验选用对数生长期长势良好的细胞，将细胞浓度调整为1×105/mL，接种于无菌的6孔板中，并分为阴性对照组（转染随机序列）、空白组及miRNA21过表达组。miRNA21过表达组采用miRNA21模拟物（miRNA21mimics）上调小鼠成纤维细胞内miRNA21的表达。使用四点弯曲细胞力学加载系统对两组细胞分别进行力学加载，建立压力性尿失禁（stress urinary incontinence, SUI）细胞模型，加力大小为0、5 333 μ strain（4 mm），加载时间设定为4 h。使用CCK8法检测各组细胞增殖，流式细胞仪检测各组细胞凋亡。结果 与对照组及miRNA21过表达组相比，加力组的细胞增殖活性明显下降，细胞凋亡率明显升高，差异有统计学意义（P<0.001）；而与加力组相比，miRNA21过表达+加力组的细胞增殖活性显著上升，细胞凋亡率明显下降，差异有统计学意义（P<0.001）。结论 miRNA21模拟物可通过上调小鼠成纤维细胞中 miRNA21的表达,影响机械力作用下小鼠成纤维细胞的增殖和凋亡，可能成为 SUI 生物治疗的新靶点。
Abstract:
ObjectiveTo determine the effect of microRNA21 mimics on the proliferation and apoptosis of mouse fibroblasts cell line L929 after mechanical strain. Methods Cultured mouse fibroblasts were divided into two groups: the control group and miRNA21overexpression group. In the miRNA21overexpression group, miRNA21 mimics were used to activate the expression of miRNA21. Cells were stretched by fourpoint bending system for 0、 5333 μ strain（4 mm）for the frequency of 01 Hz and 4 hours. The cell proliferation was detected by CCK8, The apoptosis rate were measured by flow cytometry. Results Compared with control group and miRNA21overexpression group, the cell proliferation activity was declined (P<0001) and the apoptosis rate was significantly increased (P<0001) in mechanical strain group; while the cell proliferation activity was remarkably enhanced (P<0001) and the apoptosis rate was significantly decreased (P<0001) in miRNA21overexpression + mechanical strain group when compared with mechanical strain group. Conclusion The miRNA21 mimics play an important role in the proliferation and apoptosis of mouse fibroblast after mechanical strain through upregulating miRNA21, which may be a promising target for biotherapy of stress urinary incontinence(SUI).

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（项目编号：81771562）；国家自然科学基金面上项目（项目编号：81971364）；国家重点研发计划（项目编号：2018YFC2002204）；湖北省自然科学基金青年项目（项目编号：2019CFB149）；武汉大学中央高校基本科研业务费专项资金科研项目（项目编号：2042018kf0066）
更新日期/Last Update: 2020-04-25