[1]徐峰,王莹,鲁晓燕,等.HIF1α、TLR 4与稽留流产妊娠滋养细胞凋亡的 关系研究[J].中国计划生育和妇产科,2020,(9):68-71.
　XU Feng,WANG Ying,LU Xiaoyan,et al.Study on the relationship between HIF1α, TLR 4 and apoptosis of trophoblasts in missed abortion[J].Chinese Journal of Family Planning & Gynecotokology,2020,(9):68-71.
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HIF1α、TLR 4与稽留流产妊娠滋养细胞凋亡的 关系研究
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2020年9期
页码:
68-71
栏目:
论著与临床
出版日期:
2020-09-25

文章信息/Info

Title:
Study on the relationship between HIF1α, TLR 4 and apoptosis of trophoblasts in missed abortion
作者:
徐峰1; 王莹1; 鲁晓燕1; 乔海风1; 施超2; 郁超3; 刘曼华1*
南通市第一人民医院,1.妇产科；2.病理科；3.检验科
Author(s):
XU Feng1; WANG Ying1; LU Xiaoyan1; QIAO Haifeng1; SHI Chao2; YU Chao3; LIU Manhua1*
1.Department of Obstetrics and Gynecology;2.Department of Pathology;3.Department of Laboratory,the First People's Hospital of Nantong City,Nantong Jiangsu 226001,P.R.China
关键词:
稽留流产; 妊娠早期; 缺氧诱导因子1; α亚基; Toll样受体4; 细胞凋亡
Keywords:
missed abortion; early pregnancy; HIF1α; alpha subunit; TLR 4; apoptosis
分类号:
R 71421
摘要:
目的研究稽留流产患者绒毛组织中缺氧诱导因子1α（hypoxia inducible factor 1α,HIF1α）、Toll样受体4（Toll like receptor 4， TLR 4）、Fas、FasL的表达、分布情况并进行相关性分析，探讨HIF1α、TLR 4与稽留流产妊娠滋养细胞凋亡的关系。方法选取2017年1~8月于南通市第一人民医院妇科就诊的40例稽留流产患者为稽留流产组，同期行人工流产手术的正常妊娠孕妇40例为对照组。负压吸宫留取胎膜绒毛组织，采用HE染色及电镜检查，观察绒毛组织结构；酶联免疫吸附试验法检测绒毛组织中HIF1α、TLR 4的浓度；免疫组织化学SP法检测绒毛组织中HIF1α、TLR 4、Fas、FasL蛋白表达情况，并进行相关性分析。结果两组HE染色、ELISA检测、Fas/FasL蛋白表达、相关性分析4个方面比较，差异均有统计学意义（P<005）;稽留流产组绒毛组织中HIF1α、TLR 4、Fas和FasL表达均高于对照组，差异有统计学意义（P<005）。结论HIFlα、TLR 4、Fas/FasL在稽留流产发生过程中均有一定作用，TLR 4NFκBHIF1α通路所导致的缺氧和炎症反应与稽留流产的发生密切相关。
Abstract:
ObjectiveTo study the expression and distribution of hypoxiainducible factor 1α(HIF１α), Tolllike receptor 4 (TLR 4), Fas, and FasL in the villi of missed abortion patients trophoblast apoptosis.MethodsFrom January to August 2017, 40 cases of missed abortion patients who were treated in the Gynecology Department of the First People's Hospital of Nantong City were selected as the missed abortion group, and 40 normal pregnant women who underwent artificial abortion at the same time were used as the control group. Negative pressure suction uterine retention of fetal membrane villi tissue, using HE staining and electron microscopy to observe the villi tissue structure; enzymelinked immunosorbent assay(ELISA) method to detect the concentration of HIF１α and TLR 4 in villi tissue; immunohistochemical SP method to detect expression of HIF１α, TLR 4, Fas, FasL protein in villi tissue, and correlation analysis.ResultsThere were significant differences in HE staining, ELISA detection, Fas/FasL protein expression and correlation analysis between the two groups (P<005).The expressions of HIF１α, TLR 4, Fas and FasL in the villi of the abortion group were higher than in the control group, and the differences were statistically significant(P<005).ConclusionHIFlα, TLR 4 and Fas/FasL all play a role in the occurrence of missed abortion. The hypoxia and inflammation caused by the TLR 4NFκBHIF1α pathway are closely related to the occurrence of missed abortion.

参考文献/References:

［1］刘芳，其木格．稽留流产患者血液和绒毛中雌激素受体a基因多态性的表达 ［J］．中华医学杂志，2012，92（9）：616619． ［2］张华坤，罗福薇，耿茜，等．252例稽留流产者绒毛细胞遗传学分析及环境因素初探 ［J］．中华医学遗传学杂志，2011，28（5）：575578． ［3］Kaponis A, Skyplas A, Zagorianakou N, et al. Coelomic cells show apoptosis via Fas/FasL system: a comparative study between healthy human pregnancies and missed miscarriages ［J］. Human Reproduction, 2008, 23(5): 11591169. ［4］孙澜栩，黄学惠，黄燕，等．稽留流产绒毛中HIF1α、VEGF和PLGF的表达及意义 ［J］．现代妇产科进展，2013，22（5）：367369, 372． ［5］Yan Fang, Shuang Yu, Ma Yuyan, et al. Association of Dll4/notch and HIF1α VEGF signaling in the angiogenesis of missed abortion ［J］. PLOS One, 2013, 8(8): e　70667. ［6］Beijar E E, Maliard C, Powell T L. Expression and subcellular localization of TLR4 in term and first trimester human placenta ［J］. Placenta, 2006, 27(2/3): 322326. ［7］Leng CH,Chen HW,Chang LS,et al. A recombinant lipoprotein containing an unsaturated fatty acid activates NFκB through the TLR2 signaling pathway and induces a differential gene profile from a synthetic lipopeptide ［J］. Molecular Immunology, 2010, 47(11/12): 20152021. ［8］Lorne E, Dupont H, Abraham E. Tolllike receptors 2 and 4: initiators of nonseptic inflammation in critical care medicine? ［J］. Intensive Care Medicine, 2010, 36(11): 18261835. ［9］He Zhifeng, Huang Xiaofeng, Ni Yanhong, et al. Functional tolllike receptor 3 expressed by oral squamous cell carcinoma induced cell apoptosis and decreased migration ［J］. Oral Surgery Oral Medicine Oral Pathology and Oral Radiology, 2014, 118(1): 92100. ［10］Doull L, Wattegedera S R, Longbottom D, et al. Late production of CXCL8 in ruminant oronasal turbinate cells in response to chlamydia abortus infection ［J］. Veterinary Immunology and Immunopathology, 2015, 168(1/2): 97102. ［11］Ping Fan, Zhang Jianjun, Bo Wang, et al. Hypoxiainducible factor1 upregulates the expression of Tolllike receptor 4 in pancreatic cancer cells under hypoxic conditions ［J］. Pancreatology, 2012, 12(2): 170178. ［12］Semenza G L. The hypoxic tumor microenvironment: A driving force for breast cancer progression ［J］. Biochimica et Biophysica Acta (BBA)  Molecular Cell Research, 2016, 1863(3): 382391.

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备注/Memo

备注/Memo:
南通市科技局科研计划项目（项目编号：MS22015117）
更新日期/Last Update: 2020-09-25