[1]李雅倩,周逸丹,陈娜,等.同源组织工程生物补片在阴道重建中的实验研究[J].中国计划生育和妇产科,2021,(7):50-54.[doi:10.3969/j.issn.1674-4020.2021.07.16]
　Experimental study on homologous tissue-engineering biological meshes in vaginal reconstruction[J].Chinese Journal of Family Planning & Gynecotokology,2021,(7):50-54.[doi:10.3969/j.issn.1674-4020.2021.07.16]
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同源组织工程生物补片在阴道重建中的实验研究()
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《中国计划生育和妇产科》[ISSN:1674-4020/CN:51-1708/R]

卷:
期数:
2021年7期
页码:
50-54
栏目:
出版日期:
2021-07-25

文章信息/Info

Title:
Experimental study on homologous tissue-engineering biological meshes in vaginal reconstruction
文章编号:
1674-4020（2021）07-050-05
作者:
李雅倩; 周逸丹; 陈娜; 朱兰
中国医学科学院 北京协和医学院 北京协和医院
关键词:
脱细胞基质材料; 生物补片; 阴道重建; 动物模型
分类号:
R713.3
DOI:
10.3969/j.issn.1674-4020.2021.07.16
文献标志码:
A
摘要:
目的：探讨猪皮来源的组织工程生物补片对实验猪阴道缺损模型的阴道重建的效果。方法：建立中国小型实验猪的阴道缺损模型并植入组织工程生物补片重建阴道。在术后1、2、4、6、8周分别取阴道全层，每次1只，其中第4周两只。手术前取其中2只实验猪的正常阴道作为对照组。对不同时间段的阴道全层进行H&E染色用来观察组织的生长情况；免疫组化检测上皮光谱角蛋白AE1/AE3、层粘连蛋白laminin和平滑肌肌动蛋白α-actin，以表征重建阴道的上皮生长、完整程度和平滑肌生长；VG染色评价胶原纤维的生成情况。结果：H&E染色可见重建阴道2周后出现新生粘膜；8周后新生阴道上皮结构与正常阴道无明显差异。AE1/AE3蛋白的免疫组化染色表明上皮组织的存在。Laminin的免疫组化染色提示新生阴道粘膜内基底膜成分的生成。VG染色和α-actin的表达结果显示阴道重建8周后可生成完整的胶原纤维和平滑肌肌束，且排列整齐。结论：同源组织工程生物补片进行阴道重建时可形成完整的阴道粘膜和平滑肌，与正常阴道结构相似。
Abstract:
Objective: To investigate the effect of a tissue-engineering biological mesh (acellular tissue matrix materials derived from the pig dermal) on vaginal reconstruction in the pig vaginal defect model. Methods: Vagina excision and vagina reconstruction by acellular tissue matrix materials (ATM) derived from the pig dermal were performed in 6 Chinese experimental miniature pigs. At week 1, 2, 4, 6, 8 after transplantation, the neovaginas were harvested and histopathological evaluation were performed. The vaginas harvested before surgery were used as control groups. Haematoxylin and eosin (H&E) staining were performed to investigate the morphological and inflammatory changes. Pancytokeratins (AE1/AE3) and laminin immumohistochemical (IHC) staining were applied to evaluate the regeneration and integrity of epithelium. The expression of α-actin was assessed to demonstrate the regeneration of smooth muscle. VG staining were performed to evaluate the growth of collagen fibers. Results: H&E staining showed that epithelialization was observed 2 weeks after transplantation. After 8 weeks, the neovaginas of P-ATM groups were similar with normal vaginas. AE1/AE3 staining showed the epithelial cells existed and were increased with time. Laminin staining showed the existence of basement membrane components in the neovaginas. VG staining and α-actin staining suggested the regeneration of collagen fiber and smooth muscles 8 weeks after transplantation. Conclusions: The whole vaginal epithelium and smooth muscles could be regenerated through homologous tissue-engineering biological meshes.

相似文献/References:

[1]李玢,朱兰,姜向阳,等.交联与非交联猪源生物补片对绵羊肌源性干细胞生长影响的研究[J].中国计划生育和妇产科,2015,(05):0.
　LI Fen,ZHU Lan,JIANG Xiang-yang,et al.[J].Chinese Journal of Family Planning & Gynecotokology,2015,(7):0.
[2]周幸知,陈锐娥,丁淼,等.盆底重建材料在女性盆腔脏器脱垂中的应用[J].中国计划生育和妇产科,2018,(8):5.

更新日期/Last Update: 2021-07-26